Increased leukotriene concentrationx in submandibular glands from rats with experimental periodontitis
Increased leukotriene concentrationx in submandibular glands from rats with experimental periodontitis
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DOI：
10.1007/s00011-009-0008-8
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发表时间：
2009-07-01
影响因子：
6.7
通讯作者：
Borda, Enri
中科院分区：
文献类型：
--
作者：
Busch, Lucila;Miozza, Valeria;Borda, Enri
在本研究中，我们研究了实验性牙周炎大鼠的炎症介质如一氧化氮、前列腺素和半胱氨酸-白三烯与粘蛋白释放与下颌下腺交感神经系统的关系。实验性牙周炎大鼠的下颌下腺。第一个实验，大鼠给予氢化可的松sc， 1 mg/kg，连续3天。所有其他实验均在未治疗大鼠的离体下颌腺中进行。颌下腺用半胱氨酸-白三烯、异丙肾上腺素、NDGA、FPL 55712、L-NMMA、Nio、Nz、AMG、吲哚美辛、DuP 697和阿替洛尔治疗。测定一氧化氮合酶活性、前列腺素和半胱氨酸-白三烯的产生及粘蛋白分泌。方差分析后采用Newman-Keuls统计检验。氢化可的松诱导牙周炎大鼠黏蛋白释放减少36.6% （P < 0.05）。结扎大鼠只有半胱氨酸-白三烯产量增加（79.2%,P < 0.001）。抑制半胱氨酸-白三烯生成或阻断白三烯受体分别使结扎大鼠腺体粘液分泌增加25.6% （P < 0.05）和37% （P < 0.01）。另一方面，培养培养基中存在半胱氨酸-白三烯可诱导颌下腺释放黏液。阿替洛尔降低24% (P < 0.05)，牙周炎大鼠半胱氨酸-白三烯升高。此外，异丙肾上腺素诱导两组的半胱氨酸-白三烯生成。在患有牙周炎的大鼠的下颌下腺中，粘蛋白释放的增加和半胱氨酸-白三烯的产生是相关的事件，两者都与交感神经系统有关。
In the present study, we investigated the relation between the inflammatory mediators such as nitric oxide, prostaglandins, and cysteinyl-leukotrienes with mucin release and the sympathetic system in submandibular glands from rats with experimental periodontitis.Submandibular glands from rats with experimental periodontitis.For the first experiment, rats were treated with hydrocortisone sc, 1 mg/kg for 3 days. All other experiments were carried out in isolated submandibular glands from untreated rats. Submandibular glands were treated with cysteinyl-leukotrienes, isoproterenol, NDGA, FPL 55712, L-NMMA, Nio, Nz, AMG, indomethacin, DuP 697 and atenolol.Nitric oxide synthase activity, prostaglandin and cysteinyl-leukotriene productions and mucin secretion were determined. The Newman-Keuls statistical test was applied after analysis of variance.In rats with periodontitis hydrocortisone-induced a 36.6% (P < 0.05) decrease in mucin release. Only cysteinyl-leukotriene production was increased in rats with ligature (79.2%, P < 0.001). Either the inhibition of cysteinyl-leukotriene production or the block of leukotriene receptor abolished the increase in mucin secretion by 25.6% (P < 0.05) and 37% (P < 0.01), respectively, in glands from rats with ligature. On the other hand, the presence of cysteinyl-leukotrienes in the incubation medium induced mucin release from submandibular glands. Atenolol diminished by 24% (P < 0.05), the increase in cysteinyl-leukotrienes observed in rats with periodontitis. Besides, isoproterenol induced cysteinyl-leukotriene production in both groups.In submandibular glands from rats with periodontitis, the increment in mucin release and cysteinyl-leukotrienes production are related events and both are associated with the sympathetic system.