IMMATURE LYMPHOCYTES-T IN HUMAN CORD BLOOD IDENTIFIED BY MONOCLONAL-ANTIBODIES - A MODEL FOR THE STUDY OF THE DIFFERENTIATION PATHWAY OF T-CELLS IN HUMANS
IMMATURE LYMPHOCYTES-T IN HUMAN CORD BLOOD IDENTIFIED BY MONOCLONAL-ANTIBODIES - A MODEL FOR THE STUDY OF THE DIFFERENTIATION PATHWAY OF T-CELLS IN HUMANS
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DOI：
10.1016/0008-8749(84)90209-0
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发表时间：
1984-01-01
影响因子：
4.3
通讯作者：
FERRANDO, ML
中科院分区：
文献类型：
--
作者：
FOA, R;GIUBELLINO, MC;FERRANDO, ML
人脐带血淋巴细胞的反应性进行了评估对一组单克隆抗体（MoAb）。脐带血中OKT 3+细胞（pan-T）的平均比例显著较低（52 . ±. 13.8%;平均值±。SD）与成人血（75 ± 0. E-玫瑰花结形成能力为50 ± 0.89%。16.3%）。脐带血中OKT 4+细胞（辅助/诱导表型）和OKT 8+细胞（抑制/细胞毒性表型）的比例均显著降低（43 . ±. 11.8% vs. 50.3 .+-. 7.4%和20 . ±. 10.3% vs. 25.6 .+-. 6.0%），而总的OKT 4/OKT 8比率与成人血液相比增加（2.87 ± 0.6%）。1.83与2.04 . ±. 0.61）。与成人血液不同，在35个脐带血样品中的30个中，观察到OKT 4+和OKT 8+细胞的总比例之间的重叠（65 ± 0.5%）。15.2%）和OKT 3+细胞（52 . 14.3%）。虽然偶尔发现少量细胞共表达两种抗原，双染色分析表明，在脐带血中的重叠主要是由于OKT 3（Leu-4）-/OKT 8（Leu-2）+细胞的比例扩大。与成人血液相比，在脐带血中发现了OKT 6+（普通胸腺细胞抗原）和OKT 10+（胸腺细胞、活化T细胞、前体细胞）细胞的相关比例（10.8 ± 0.001）。8.6% vs. 0.6 .+-. 0.6%和67 . 18.0% vs. 8 .+-. 2.1%），而末端脱氧核苷酸转移酶阳性细胞仅在2份脐带血样品中观察到。一小部分T细胞（E玫瑰花结+）与MoAb OKIa 1（HLA-DR）反应。最后，与成人血液相比，被MoAb Leu-7（HNK-1克隆）识别的脐带血细胞的比例几乎可以忽略不计（2.8 ± 0.5）。2.4% vs. 15 .+-. 7.5%）。这些数据证实了脐带血淋巴细胞的不成熟性和异质性，并证明了出生时存在循环淋巴细胞，其表达的表面表型让人想起胸腺内分化晚期和某些人T细胞白血病中发现的表型。因此，人脐带血可以代表用于研究人类正常和病理性T细胞的分化途径的合适模型。
The reactivity of human cord blood lymphocytes was assessed against a panel of monoclonal antibodies (MoAb). The mean proportion of OKT3+ cells (pan-T) was significantly lower in cord blood (52 .+-. 13.8%; mean .+-. SD) compared with that of adult blood (75 .+-. 8.9%) and paralleled well with the E-rosette-forming capacity (50 .+-. 16.3%). Both the proportions of OKT4+ cells (helper/inducer phenotype) and of OKT8+ cells (suppressor/cytotoxic phenotype) were significantly reduced in cord blood (43 .+-. 11.8% vs. 50.3 .+-. 7.4% and 20 .+-. 10.3% vs. 25.6 .+-. 6.0%, respectively), while the overall OKT4/OKT8 ratio was increased compared with adult blood (2.87 .+-. 1.83 vs. 2.04 .+-. 0.61). Unlike adult blood, in 30 of the 35 samples of cord blood an overlap was observed between the total proportion of OKT4+ and OKT8+ cells (65 .+-. 15.2%) and that of OKT3+ cells (52 .+-. 14.3%). Although small numbers of cells coexpressing both antigens were occasionally found, double-staining analysis showed that the overlap in cord blood was mostly due to an expanded proportion of OKT3 (Leu-4)-/OKT8 (Leu-2)+ cells. Relevant proportions of OKT6+ (common thymocyte antigen) and OKT10+ (thymocytes, activated T cells, precursor cells) cells were found in cord blood as opposed to adult blood (10.8 .+-. 8.6% vs. 0.6 .+-. 0.6% and 67 .+-. 18.0% vs. 8 .+-. 2.1%, respectively), while terminal deoxynucleotidyl transferase-positive cells were observed only in 2 samples of cord blood. A small proportion of T cells (E-rosette+) reacted with the MoAb OKIa1 (HLA-DR). Finally, the proportion of cord blood cells recognized by the MoAb Leu-7 (HNK-1 clone) was almost negligible compared with adult blood (2.8 .+-. 2.4% vs. 15 .+-. 7.5%). These data confirm the immaturity and heterogeneity of cord blood lymphocytes and demonstrate the presence at birth of circulating lymphocytes which express a surface phenotype reminiscent of that found in the late stages of intrathymic differentiation and in some human T-cell leukemias. Human cord blood may thus represent a suitable model for the study of the differentiation pathway of normal and pathological T cells in humans.