Microwaves as modulators of membrane stability parameters during hepatic cancer
Microwaves as modulators of membrane stability parameters during hepatic cancer
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微波作为肝癌期间膜稳定性参数的调节剂
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通讯作者：
Wang Qiucheng
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作者：
Guo cunli;Bi yumei;Zhang Lei;Xu Yi;Wang Qiucheng
目的：探讨微波对二乙基亚硝胺（DEN）诱导的肝细胞癌（HCC）细胞膜流动性变化的影响。方法：将大鼠分为正常对照组、DEN处理组、微波处理组、DEN+微波处理组。从大鼠身上分离出刷状边界膜（BBM），利用膜外荧光基团芘对其粘度和流动性参数进行了测定。结果：DEN治疗导致脂质过氧化（LPO）显著升高。DEN处理显著降低了谷胱甘肽水平（GSH）以及谷胱甘肽还原酶（GR）、谷胱甘肽转移酶（GST）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。另一方面，微波处理den处理大鼠的脂质过氧化水平显著降低，但GSH水平显著升高，GR、GST、SOD、CAT和GPx活性显著升高。结果进一步表明，DEN处理后膜微粘度显著降低。另一方面，与正常对照大鼠相比，den处理大鼠的准分子/单体比和流动性参数明显增加。微波处理后，膜微粘度和流动性参数的变化明显恢复。结论：微波对den诱导肝癌后细胞膜稳定性参数的调节具有重要作用。
Purpose: The present study explored the potential of microwaves on membrane fluidity changes in diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC), in vivo...Methods: Rats were segregated into four groups: normal control, DEN-treated, microwave-treated, DEN+microwave-treated. Brush border membranes (BBM) were isolated from the rats and, using the membrane extrinsic fluorophore pyrene, we assessed the viscosities as well as fluidity parameters. ..Results: DEN treatment resulted in a significant rise in lipid peroxidation (LPO). Reduced glutathione levels (GSH) and the activities of glutathione reductase (GR), glutathione transferase (GST), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were found to be significantly decreased following DEN treatment. On the other hand, microwave treatment in DEN-treated rats resulted in a significant decrease in the levels of lipid peroxidation but caused a significant rise in the levels of GSH as well in the activities of GR, GST, SOD, CAT and GPx. The results further demonstrated a marked decrease in membrane microviscosity following DEN treatment. On the other hand, a significant increase was observed in the excimer/monomer ratio and fluidity parameter of DEN-treated rats when compared to normal control rats. However, the alterations in membrane microviscosity and the fluidity parameters were significantly restored after microwave treatment. ..Conclusion: The study, therefore, concludes that microwave proved quite useful in the modulation of membrane stability parameters following DEN-induced hepatic cancer.