Identification of a third human polyomavirus
Identification of a third human polyomavirus
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DOI：
10.1128/jvi.00028-07
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发表时间：
2007-04-01
影响因子：
5.4
通讯作者：
Andersson, Bjorn
中科院分区：
文献类型：
--
作者：
Allander, Tobias;Andreasson, Kalle;Andersson, Bjorn
我们以前曾报道过一种用于临床样本大规模分子病毒筛选的系统。作为系统搜索未被识别的人类病原体工作的一部分，该技术已应用于人类呼吸道样本的病毒筛选。这导致鉴定出一种以前未知的多瘤病毒，暂时命名为KI多瘤病毒。该病毒在系统发育上与基因组早期区域的其他灵长类多瘤病毒相关，但与基因组后期区域的已知多瘤病毒几乎没有同源性（氨基酸同源性< 30%）。通过聚合酶链反应（PCR）在637份鼻咽吸入物中的6份（1%）和192份粪便样本中的1份（0.5%）中发现了该病毒，但在几组尿液和血液样本中未检测到该病毒。由于多瘤病毒具有致癌潜力，并可能在免疫抑制个体中产生严重疾病，因此在不同的医学背景下继续寻找该病毒非常重要。这一发现进一步说明了呼吸道样本的无偏筛选如何用于发现不同类型的病毒。
We have previously reported on a system for large-scale molecular virus screening of clinical samples. As part of an effort to systematically search for unrecognized human pathogens, the technology was applied for virus screening of human respiratory tract samples. This resulted in the identification of a previously unknown polyomavirus provisionally named KI polyomavirus. The virus is phylogenetically related to other primate polyomaviruses in the early region of the genome but has very little homology (< 30% amino acid identity) to known polyomaviruses in the late region. The virus was found by PCR in 6 (1%) of 637 nasopharyngeal aspirates and in 1 (0.5%) of 192 fecal samples but was not detected in sets of urine and blood samples. Since polyomaviruses have oncogenic potential and may produce severe disease in inummosuppressed individuals, continued searching for the virus in different medical contexts is important. This finding further illustrates how unbiased screening of respiratory tract samples can be used for the discovery of diverse virus types.