Disrupting Rb-Raf-1 interaction inhibits hair cell regeneration in zebrafish lateral line neuromasts
Disrupting Rb-Raf-1 interaction inhibits hair cell regeneration in zebrafish lateral line neuromasts
复制标题
破坏 Rb-Raf-1 相互作用抑制斑马鱼侧线神经丘毛细胞再生
DOI：
10.1097/wnr.0b013e32835e3279
复制
发表时间：
2013-03-06
期刊：
影响因子：
1.7
通讯作者：
Li, Huawei
中科院分区：
文献类型：
--
作者：
Lin, Qin;Li, Wenyan;Li, Huawei
斑马鱼神经肥大是研究耳毒性损伤后毛细胞（HC）死亡和再生的理想模型。新霉素损伤后，斑马鱼幼鱼体内HC发生快速而稳健的替换。然而，新碳氢化合物的来源仍不清楚。我们的数据表明，不对称的细胞分裂参与了斑马鱼侧线神经瘤的HC再生过程。此外，小分子RRD 251，破坏RB和Raf-1之间的物理相互作用，然后阻断Rb的磷酸化，可以抑制HC再生支持细胞增殖。我们的研究结果表明，Rb-Raf-1相互作用在斑马鱼的自发HC再生中起着重要作用。NeuroReport 24：190-195（C）2013年威科健康垂直酒吧Lippincott威廉姆斯&威尔金斯。神经报告2013，24：190-195
Zebrafish neuromast is an ideal model for investigating hair cell (HC) death and regeneration following ototoxic insults. HC undergoes rapid and robust replacement in larval zebrafish after neomycin damage. However, the origin of new HCs remains unclear. Our data showed that asymmetric cell division was involved in the process of HC regeneration in zebrafish lateral line neuromasts. Furthermore, a small molecule RRD251, which disrupted the physical interaction between RB and Raf-1 and then blocked the phosphorylation of Rb, could have inhibited the HC regeneration from supporting cell proliferation. Our results indicate that Rb-Raf-1 interaction plays an important role in spontaneous HC regeneration in zebrafish. NeuroReport 24:190-195 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. NeuroReport 2013, 24: 190-195