Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum
Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum
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DOI：
10.1074/jbc.m110.118554
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发表时间：
2010-09-03
影响因子：
4.8
通讯作者：
Dorrestein, Pieter C.
中科院分区：
文献类型：
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作者：
Gonzalez, David J.;Lee, Shaun W.;Dorrestein, Pieter C.
通过精制肉毒杆菌毒素，肉毒梭菌会产生婴儿肉毒杆菌中毒、伤口肉毒杆菌中毒和其他侵袭性感染的临床综合征。通过比较基因组分析，在肉毒梭菌和相关食源性病原体产孢梭菌中发现了一个孤儿九基因簇，该簇类似于链球菌溶血素 S 的生物合成机制，链球菌溶血素 S 是 A 组链球菌的关键毒力因子，导致其标志性 β 溶血表型。遗传互补、体外重建、质谱分析和质粒整合诱变证明来自梭状芽胞杆菌的链球菌溶血素 S 样基因簇。负责一种新型翻译后修饰的溶血毒素梭菌溶血素 S 的生物发生。
Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark beta-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.