RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis
RING-finger protein 5 promotes hepatocellular carcinoma progression and predicts poor prognosis
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DOI：
10.1007/s13577-020-00460-5
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发表时间：
2021-01-19
期刊：
影响因子：
4.3
通讯作者：
Wang, Xiaomin
中科院分区：
文献类型：
--
作者：
Zhang, Yi;Li, Jie;Wang, Xiaomin
肝细胞癌（HCC）是恶性肿瘤相关死亡的最常见原因之一。最近，环指蛋白5（RNF 5）（一种E3遍在蛋白连接酶）被发现与多种人类癌症的发生有关。然而，RNF5在HCC中的临床意义和功能作用知之甚少。我们分析了肝癌样本中RNF5的表达，并观察到RNF5的mRNA和蛋白水平在肝癌组织中均显著增加。RNF5的上调与较大的肿瘤大小、更多的卫星灶和较高的甲胎蛋白（AFP）水平显著相关，表明HCC患者预后不良。敲低和过表达实验表明RNF5在体外促进肝癌细胞的增殖、迁移和侵袭。此外，RNF 5促进体内肝癌生长。我们的研究结果表明，RNF5是HCC进展的癌基因，可作为HCC患者的新的预后生物标志物和治疗靶点。
Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related deaths. Recently, RING-finger protein 5 (RNF5), an E3 ubiquitin ligase, was revealed to be associated with the development of several human cancers. However, the clinical implication and functional role of RNF5 in HCC are poorly understood. We analysed RNF5 expression in HCC samples and observed that both the mRNA and protein levels of RNF5 were significantly increased in HCC tissues. RNF5 upregulation was markedly associated with larger tumour size, more satellite foci, and higher alpha fetoprotein (AFP) level, indicating poor prognosis in patients with HCC. Knockdown and overexpression experiments demonstrated that RNF5 promoted the proliferation, migration, and invasion of HCC cells in vitro. Moreover, RNF5 facilitated HCC growth in vivo. Our findings indicated that RNF5 was an oncogene of HCC progression and could be used as a novel prognostic biomarker and therapeutic target for patients with HCC.