A New Family of Stoneflies (Insecta: Plecoptera), Kathroperlidae, fam. n., with a Phylogenomic Analysis of the Paraperlinae (Plecoptera: Chloroperlidae)
A New Family of Stoneflies (Insecta: Plecoptera), Kathroperlidae, fam. n., with a Phylogenomic Analysis of the Paraperlinae (Plecoptera: Chloroperlidae)
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DOI：
10.1093/isd/ixab014
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发表时间：
2021-07
影响因子：
3.4
通讯作者：
Eric J. South-;R. Skinner;R. DeWalt;Mark A. Davis;K. Johnson;V. A. Teslenko;Jonathan J. Lee;Rachel L. Malison;J. Hwang;Y. Bae;L. Myers
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文献类型：
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作者：
Eric J. South-;R. Skinner;R. DeWalt;Mark A. Davis;K. Johnson;V. A. Teslenko;Jonathan J. Lee;Rachel L. Malison;J. Hwang;Y. Bae;L. Myers
摘要最近对北美直翅目92属94种昆虫的转录组数据进行了分子分析，确定Kathroperla Banks，1920属为斑潜蝇科+斑潜蝇科的姊妹群。鉴于Kathroperla属在历史上一直被列为Chloroplidae科的成员，这一发现表明需要对该属和Parperlinae亚科进行进一步的调查。转录组和基因组测序数据集都来自于32种亚目的Systellognatha，包括所有已描述的Parperlinae种，以测试这些分类群的系统发育位置。从这些数据集中，产生了800个同源基因的大型系统基因组数据矩阵，并进行了多种分析，包括串联和合并分析。在光学显微镜下对所有副线虫的形态进行了比较。所有的分子结果都支持Kathroperla是一个单系分类群，六个分子分析中有五个支持Kathroperla作为其余Perloidae的姊妹分类群。眼后头长被确定为该属的一个明显的形态特征。综合的分子和形态证据支持Kathroperlidae，fam的命名。N.，为现存的长翅目第17科。
Abstract Recent molecular analyses of transcriptome data from 94 species across 92 genera of North American Plecoptera identified the genus Kathroperla Banks, 1920 as sister group to Chloroperlidae + Perlodidae. Given that the genus Kathroperla has historically been included as a member of the family Chloroperlidae, this discovery indicated further investigation of the genus and the subfamily Paraperlinae was needed. Both transcriptome and genome sequencing datasets were generated from 32 species of the infraorder Systellognatha, including all described species of the Paraperlinae, to test the phylogenetic placement of these taxa. From these datasets, a large phylogenomic data matrix of 800 orthologous genes was produced, and multiple analyses were conducted, including both concatenated and coalescent analyses. Morphological comparisons were made among all Paraperlinae using light microscopy. All molecular results support a monophyletic Kathroperla, which is supported as sister taxon to the remaining Perloidea by five of six molecular analyses. Postocular head length is determined to be a distinct morphological character of this genus. Combined molecular and morphological evidence support the designation of Kathroperlidae, fam. n., as the seventeenth family of extant Plecoptera.