High-strength silk protein scaffolds for bone repair
High-strength silk protein scaffolds for bone repair
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DOI：
10.1073/pnas.1119474109
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发表时间：
2012-05-15
影响因子：
11.1
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
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作者：
Mandal, Biman B.;Grinberg, Ariela;Kaplan, David L.
用于骨组织再生的生物材料是骨科研究的主要焦点。然而，今天只有少数聚合物生物材料被利用，因为它们未能解决关键问题，如承重骨移植物的抗压强度。在这项研究中，高压缩强度（类似于13 MPa的水合状态）聚合物骨复合材料的发展报告，基于丝蛋白-蛋白质界面结合。将利用碱水解获得的10-600 μ m大小的丝纤维用作致密纤维复合材料中的增强材料，所述纤维复合材料具有可调的压缩强度、表面粗糙度和基于所包括的纤维长度的孔隙率。表面粗糙度、孔隙率和支架刚度的组合有利于人骨髓间充质干细胞在体外基于骨标记物的生化和基因表达向骨样组织分化。此外，最小的体内免疫调节反应表明，制造的丝纤维增强复合材料基质的骨工程应用的兼容性。
Biomaterials for bone tissue regeneration represent a major focus of orthopedic research. However, only a handful of polymeric biomaterials are utilized today because of their failure to address critical issues like compressive strength for load-bearing bone grafts. In this study development of a high compressive strength (similar to 13 MPa hydrated state) polymeric bone composite materials is reported, based on silk protein-protein interfacial bonding. Micron-sized silk fibers (10-600 mu m) obtained utilizing alkali hydrolysis were used as reinforcement in a compact fiber composite with tunable compressive strength, surface roughness, and porosity based on the fiber length included. A combination of surface roughness, porosity, and scaffold stiffness favored human bone marrow-derived mesenchymal stem cell differentiation toward bone-like tissue in vitro based on biochemical and gene expression for bone markers. Further, minimal in vivo immunomodulatory responses suggested compatibility of the fabricated silk-fiber-reinforced composite matrices for bone engineering applications.