Response to Comment on "Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies": A Comment on "How a phase image of a cell with nucleus refractive index smaller than that o
Response to Comment on "Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies": A Comment on "How a phase image of a cell with nucleus refractive index smaller than that o
复制标题
对“核折射率是否低于细胞质？相位测量的验证及其对光散射技术的影响”的评论的回应：对“核折射率小于细胞质的细胞的相位图像如何”的评论
DOI：
10.1002/jbio.201800091
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发表时间：
2018
影响因子：
2.8
通讯作者：
Wax,Adam
中科院分区：
文献类型：
--
作者：
Steelman,ZacharyA;Eldridge,WillJ;Wax,Adam
最近，Maxim A. Yurkin对我们的论文《核折射率比细胞质低吗？》验证相位测量和光散射技术的意义”，以及sch<s:1> rmann等人的补充研究“细胞核的折射率和质量密度比细胞质低”。在他的评论中，Yurkin得出结论，光学密度小于细胞质的细胞核的定量相图像必须表现出特征性的凹面，缺乏凹面的证据与我们的结论相反。在这个回应中，我们认为Yurkin的结论是通过过度简化细胞内的空间折射率分布得出的，这并没有考虑到高折射率的包涵体，如核仁。我们进一步引用了最近在三维折射率成像方面的研究，这些研究的优势支持我们的结论。最后，我们评论了目前关于活细胞亚细胞折射率分布的知识状况。
Recently, Maxim A. Yurkin commented on our paper “Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies” as well as on a complementary study “Cell nuclei have lower refractive index and mass density than cytoplasm” from Schürmann et al. In his comment, Yurkin concluded that quantitative phase images of cells with nuclei that are less optically dense than the cytoplasm must exhibit a characteristic concavity, the absence of which is evidence against our conclusion of a less‐dense nucleus. In this response, we suggest that Yurkin's conclusion is reached through an oversimplification of the spatial refractive index distribution within cells, which does not account for high index inclusions such as the nucleolus. We further cite recent studies in 3‐dimensional refractive index imaging, in which the preponderance of studies supports our conclusion. Finally, we comment on the current state of knowledge regarding subcellular refractive index distributions in living cells.