Dopamine transporter (DAT) and synaptic vesicle amine transporter (VMAT2) gene expression in the substantia nigra of control and Parkinson's disease
Dopamine transporter (DAT) and synaptic vesicle amine transporter (VMAT2) gene expression in the substantia nigra of control and Parkinson's disease
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DOI：
10.1016/0169-328x(95)00278-z
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Emson, PC
中科院分区：
文献类型：
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作者：
Harrington, KA;Augood, SJ;Emson, PC
使用原位杂交技术，在对照和帕金森病组织中的人死后黑质切片中研究了 DAT mRNA 和 VMAT2 mRNA 的细胞表达。使用短的合成寡脱氧核苷酸在细胞水平上检测这些基因转录本。在对照人类黑质中，在黑质的所有分区中都观察到高水平的表达，特别是在内侧区域内。相比之下，帕金森病中 DAT mRNA 和 VMAT2 mRNA 的表达水平均显着降低；这些杂交信号的减少与（i）黑质中含多巴胺细胞的显着损失，以及（ii）剩余色素神经元中每个细胞的 DAT 和 VMAT2 信号减少有关。帕金森病黑质幸存细胞中 DAT 和 VMAT2 基因转录本的细胞丰度与疾病相关的减少可能反映了儿茶酚胺信号传导的代偿性变化，也可能是神经元功能障碍的结果。
The cellular expression of DAT mRNA and VMAT2 mRNA was investigated in sections of the human post-mortem substantia nigra in control and Parkinson's disease tissue using in situ hybridisation techniques. Short synthetic oligodeoxynucleotides were used to detect these gene transcripts at the cellular level. In the control human nigra, high levels of expression were seen in all sub-divisions of the substantia nigra, especially within medial regions. By contrast, the level of expression of both DAT mRNA and VMAT2 mRNA was markedly reduced in Parkinson's disease; these reductions in hybridisation signal were associated with (i) a marked loss of dopamine-containing cells in the substantia nigra, and (ii) a reduction in both DAT and VMAT2 signal per cell in the remaining pigmented neurones. These disease-related decreases in the cellular abundance of both DAT and VMAT2 gene transcripts in the surviving cells of the parkinsonian nigra may reflect compensatory changes in catecholamine signalling or may be a consequence of neuronal dysfunction.