Disruption of Biofilm Formation by the Human Pathogen Acinetobacter baumannii Using Engineered Quorum-Quenching Lactonases
Disruption of Biofilm Formation by the Human Pathogen Acinetobacter baumannii Using Engineered Quorum-Quenching Lactonases
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DOI：
10.1128/aac.02410-13
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发表时间：
2014-03-01
影响因子：
4.9
通讯作者：
Yew, Wen Shan
中科院分区：
文献类型：
--
作者：
Chow, Jeng Yeong;Yang, Yuanyong;Yew, Wen Shan
鲍曼不动杆菌是一种与多重耐药医院感染相关的主要人类病原体；其毒力归因于群体感应介导的生物膜形成，而破坏生物膜形成是一种有吸引力的抗毒策略。在这里，我们报告了使用通过定向进化获得的群体淬灭内酯酶首次成功演示了鲍曼不动杆菌 S1 临床分离株的生物膜破坏；这种工程内酯酶显着降低了鲍曼不动杆菌相关生物膜的生物量，证明了这种抗毒策略的实用性。
Acinetobacter baumannii is a major human pathogen associated with multidrug-resistant nosocomial infections; its virulence is attributed to quorum-sensing-mediated biofilm formation, and disruption of biofilm formation is an attractive antivirulence strategy. Here, we report the first successful demonstration of biofilm disruption in a clinical isolate of A. baumannii S1, using a quorum-quenching lactonase obtained by directed evolution; this engineered lactonase significantly reduced the biomass of A. baumannii-associated biofilms, demonstrating the utility of this antivirulence strategy.