Gut microbiota composition and bone mineral lossepidemiologic evidence from individuals in Wuhan, China
Gut microbiota composition and bone mineral lossepidemiologic evidence from individuals in Wuhan, China
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中国武汉地区个体肠道菌群组成和骨矿物质损失的流行病学证据
DOI：
10.1007/s00198-019-04855-5
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发表时间：
2019-05-01
影响因子：
4
通讯作者：
Wang, Q.
中科院分区：
文献类型：
--
作者：
Li, C.;Huang, Q.;Wang, Q.
通过高通量16S核糖体RNA(RRNA)基因测序，我们探索了中国老年人肠道微生物区系组成与骨矿物质丢失的关系。与对照组相比，低骨密度组的操作分类单位(OTU)较少，多个类群的丰度发生变化，并存在特定的功能途径。方法收集102份符合条件的低骨密度个体和对照组的粪便样本，进行高通量16S rRNA基因测序。结果低骨密度个体的OTU和细菌分类群数量均较少。在门水平上，低骨密度组类杆菌较多，对照组类杆菌较丰富，类杆菌与放线菌呈正相关，类杆菌与骨密度、T评分呈负相关。在科水平上，低BMD个体的Lachnospiraceae丰度降低，且与BMD和T-Score呈正相关；同时，BMD随双歧杆菌科的增加而增加。在属水平上，低骨密度个体的月季花比例低于对照个体(P
The SummaryWe explored the association between gut microbiota composition and bone mineral loss in Chinese elderly people by high-throughput 16S ribosomal RNA (rRNA) gene sequencing. Compared with controls, a smaller number of operational taxonomic units (OTUs), several taxa with altered abundance, and specific functional pathways were found in individuals with low-bone mineral density (BMD).IntroductionGut microbiota plays important roles in human health and associates with a number of diseases. However, few studies explored its association with bone mineral loss in human.MethodsWe collected 102 fecal samples from each eligible individual belonging to low-BMD and control groups for high-throughput 16S rRNA gene sequencing.ResultsThe low-BMD individuals had a smaller number of OTUs and bacterial taxa at each level. At the phylum level, Bacteroidetes were more abundant in the low-BMD group; Firmicutes were enriched in the control group; Firmicutes and Actinobacteria positively correlated and Bacteroidetes negatively correlated with the BMD and T-score in all subjects. At the family level, the abundance of Lachnospiraceae in low-BMD individuals reduced and positively correlated with BMD and T-score; meanwhile, BMD increased with increasing Bifidobacteriaceae. At the genus level, low-BMD individuals had decreased proportions of Roseburia compared with control ones (P