Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
Kidney Expression of Toll Like Receptors in Lupus Nephritis: Quantification and Clinicopathological Correlations
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DOI：
10.1155/2016/7697592
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发表时间：
2016-01-01
影响因子：
4.6
通讯作者：
Valesini, Guido
中科院分区：
文献类型：
--
作者：
Conti, Fabrizio;Spinelli, Francesca Romana;Valesini, Guido
目标。本研究旨在定位和定量Toll样受体（TLR） 3、7、8和9在狼疮肾炎（LN）患者肾脏中的表达，并将其与临床病理特征联系起来。方法。采用抗人TLR3、TLR7、TLR8和TLR9多克隆抗体对26例LN患者和4例对照者的肾脏切片进行免疫组化分析；在数字化图像上评估tlr阳性核数/mm(2)。结果。与对照组相比，LN显示肾小球和小管间质TLR9 （p = 0.003和p = 0.007）、全肾和小管间质TLR3 （p = 0.026和p = 0.031）和高肾小管间质TLR7 （p = 0.022）的含量显著升高。TLR9与活动指数呈正相关（p = 0.0063）， TLR7与慢性指数呈正相关（p = 0.026）。TLR9与肾- sledai呈正相关（p = 0.01）。结论。这是第一个量化LN患者肾中tlr表达的研究；结果显示TLR3、TLR7和TLR9过表达，并与临床病理指标相关，支持这些介质在LN发病机制中的作用。
Objective. The study aimed at locating and quantifying Toll Like Receptor (TLR) 3, 7, 8, and 9 expression in kidney of patients with lupus nephritis (LN) and correlating them with clinicopathological features. Methods. Kidney sections from 26 LN patients and 4 controls were analyzed by immunohistochemistry using anti-human TLR3, TLR7, TLR8, and TLR9 polyclonal antibodies; the number of TLR-positive nuclei/mm(2) was evaluated on digitalized images. Results. Compared to controls, LN showed a significantly higher amount of glomerular and tubulointerstitial TLR9 (p = 0.003 and p = 0.007), whole and tubulointerstitial TLR3 (p = 0.026 and p = 0.031), and a higher tubulointerstitial TLR7 (p = 0.022). TLR9 positively correlated with activity index (p = 0.0063) and tubular TLR7 with chronicity index (p = 0.026). TLR9 positively correlated with Renal-SLEDAI (p = 0.01). Conclusions. This is the first study quantifying kidney expressions of TLRs in LN patients; the results show an overexpression of TLR3, TLR7, and TLR9 and demonstrate a correlation with clinicopathological indices supporting a role of these mediators in the pathogenesis of LN.