Sequence analysis and expression of an X-linked, lymphocyte-regulated gene family (XLR).
Sequence analysis and expression of an X-linked, lymphocyte-regulated gene family (XLR).
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DOI：
10.1084/jem.166.6.1702
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发表时间：
1987-12-01
期刊：
影响因子：
--
通讯作者：
Cohen DI
中科院分区：
文献类型：
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作者：
Siegel JN;Turner CA;Klinman DM;Wilkinson M;Steinberg AD;MacLeod CL;Paul WE;Davis MM;Cohen DI
XLR基因家族由大约10个X连锁基因组成，其表达在淋巴细胞发育中受到调节。该基因家族的某些成员与鼠xid免疫缺陷突变密切相关。来自浆细胞瘤MOPC 167的cDNA克隆pM1的序列分析显示，开放阅读框架能够编码208个氨基酸和分子量24，000的蛋白质。信号肽或跨膜区的缺乏表明预测的pM1蛋白可能位于细胞质或细胞核内。预测的蛋白质与核纤层蛋白A和C以及中间丝蛋白家族的其他成员具有显着的同源性，并且对于这些蛋白质提出的卷曲螺旋结构具有重要的特征。来自于一个前分泌淋巴瘤和成人胸腺的cDNA克隆的分析表明，B和T淋巴细胞转录一个共同的主要mRNA相同的pM1，而其他罕见的成绩单也确定了这些研究。一系列代表胸腺分化不同阶段的克隆性T淋巴瘤细胞系表明，与B淋巴肿瘤一样，XLR表达与胸腺瘤的成熟相关。
The XLR gene family consists of approximately 10 X-linked genes, the expression of which is regulated in lymphocyte development. Certain members of the gene family are closely linked to the murine xid immune deficiency mutation. Sequence analysis of a cDNA clone pM1 derived from the plasmacytoma MOPC167 showed an open reading frame capable of coding for a protein of 208 amino acids and mol wt 24,000. The lack of a signal peptide or transmembrane region indicates a probable cytoplasmic or nuclear localization for the predicted pM1 protein. The predicted protein shares significant homology with lamins A and C and other members of the intermediate filament family of proteins, and shares features important for the coiled-coil structure proposed for these proteins. Analysis of cDNA clones derived from a presecretory lymphoma and from adult thymus indicates that B and T lymphocytes transcribe a common major mRNA identical to pM1, while other rare transcripts were also identified by these studies. A series of clonal T lymphoma lines representing distinct stages of thymic differentiation showed that, as with B lymphoid tumors, XLR expression is correlated with the maturation of the thymomas.