Progastrin-like immunoreactivity in porcine antrum: identification and characterization with region-specific antisera.
Progastrin-like immunoreactivity in porcine antrum: identification and characterization with region-specific antisera.
复制标题
猪窦中的前胃泌素样免疫反应性：用区域特异性抗血清进行鉴定和表征。
DOI：
10.1016/0006-291x(85)90572-8
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发表时间：
1985
影响因子：
3.1
通讯作者：
Yamada,T
中科院分区：
文献类型：
--
作者：
Sugano,K;Yamada,T
为了鉴定和表征组织中胃泌素的前体，我们根据胃泌素mRNA的核苷酸序列，生成了针对合成的前胃泌素肽tyr - gly - trp - met - asp - ph - gly - arg - arg （GL9）的区域特异性抗血清。该抗血清不与具有较短羧基末端延伸的胃泌素或前胃泌素肽交叉反应。测定猪胃窦中原胃泌素样免疫反应性（PGLI），浓度为6.8±1.2 pmol/g湿重（平均值±SE， n = 5），约为胃泌素的0.2%。在Sephadex G50层析上，PGLI的一个主峰被洗脱为比胃泌素七肽（G17）稍大的分子，但具有相同的n端免疫反应性。这些发现表明，G17可能是通过加工羧基末端延伸的前体来替代大胃泌素的切割而形成的（G34）。
In an effort to identify and characterize precursors of gastrin in tissues, we generated region-specific antisera against a synthetic progastrin peptide, Tyr-Gly-Trp-Met-Asp-Phe-Gly-Arg-Arg (GL9), as deduced from the nucleotide sequence of gastrin mRNA. This antisera did not cross-react with gastrin or progastrin peptides with shorter carboxyl-terminal extensions. Progastrin-like immunoreactivity (PGLI) was measured in porcine antrum at a concentration of 6.8 ± 1.2 pmol/g wet weight (mean ± SE, n = 5), or roughly 0.2% of that of gastrin. On Sephadex G50 chromatography, a major peak of PGLI was eluted as a slightly larger molecule than gastrin heptadecapeptide (G17) but possessed the same N-terminal immunoreactivity. These findings suggest that G17 may be formed by processing of a carboxyl-terminally extended precursor as an alternative to cleavage of big gastrin (G34).