Effects of Chlorhexidine mouthwash on the oral microbiome
Effects of Chlorhexidine mouthwash on the oral microbiome
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DOI：
10.1038/s41598-020-61912-4
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发表时间：
2020-03-24
影响因子：
4.6
通讯作者：
Hickson, Mary
中科院分区：
文献类型：
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作者：
Bescos, Raul;Ashworth, Ann;Hickson, Mary
在单盲、交叉和非随机设计后，我们研究了7天使用氯己定（CHX）漱口水对36名健康个体唾液微生物组以及几种唾液和血浆生物标志物的影响。他们用安慰剂漱口水每天漱口两次（1分钟），持续7天，然后用CHX漱口水重复该方案，持续7天。在每次治疗结束时采集唾液和血液样本，以分析口腔细菌的丰度和多样性，以及pH值，乳酸盐，葡萄糖，硝酸盐和亚硝酸盐浓度。CHX显著增加了厚壁菌门和变形菌门的丰度，降低了拟杆菌门、TM 7、SR 1和梭菌门的含量。这种转变与唾液pH值和缓冲能力的显著降低有关，同时伴有唾液乳酸盐和葡萄糖水平的增加。使用CHX后发现唾液和血浆亚硝酸盐浓度较低，随后出现收缩压升高的趋势。总的来说，这项研究表明，含有CHX的漱口水与唾液微生物组的重大变化有关，导致健康个体的酸性条件更强，亚硝酸盐可用性更低。
Following a single blind, cross-over and non-randomized design we investigated the effect of 7-day use of chlorhexidine (CHX) mouthwash on the salivary microbiome as well as several saliva and plasma biomarkers in 36 healthy individuals. They rinsed their mouth (for 1 min) twice a day for seven days with a placebo mouthwash and then repeated this protocol with CHX mouthwash for a further seven days. Saliva and blood samples were taken at the end of each treatment to analyse the abundance and diversity of oral bacteria, and pH, lactate, glucose, nitrate and nitrite concentrations. CHX significantly increased the abundance of Firmicutes and Proteobacteria, and reduced the content of Bacteroidetes, TM7, SR1 and Fusobacteria. This shift was associated with a significant decrease in saliva pH and buffering capacity, accompanied by an increase in saliva lactate and glucose levels. Lower saliva and plasma nitrite concentrations were found after using CHX, followed by a trend of increased systolic blood pressure. Overall, this study demonstrates that mouthwash containing CHX is associated with a major shift in the salivary microbiome, leading to more acidic conditions and lower nitrite availability in healthy individuals.