Effect of dietary medium-chain fatty acids on nursery pig growth performance, fecal microbial composition, and mitigation properties against porcine epidemic diarrhea virus following storage
Effect of dietary medium-chain fatty acids on nursery pig growth performance, fecal microbial composition, and mitigation properties against porcine epidemic diarrhea virus following storage
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DOI：
10.1093/jas/skz358
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Burkey, Thomas E.
中科院分区：
文献类型：
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作者：
Gebhardt, Jordan T.;Thomson, Katelyn A.;Burkey, Thomas E.
本试验旨在研究日粮中添加中链脂肪酸（MCFA）对保育猪生长性能、粪便微生物组成和贮藏后猪流行性腹泻病毒（PEDV）的影响。在断奶当天（约20日龄），将总计360头猪（DNA 400 x 200，哥伦布，NE;最初为6.7 +/- 0.07 kg）随机分配至畜栏（每栏5头猪），允许6天适应，按BW分组，并随机分配至饮食处理（每处理9个畜栏）。所有MCFA（Sigma-Aldrich，St. Louis，MO）都保证纯度>= 98%，包括己酸（C6：0）、辛酸（C8：0）和癸酸（C10：0）。处理饲料分2个阶段（7至11 kg BW和11至23 kg BW）配制，并配制以满足或超过NRC要求估计值。处理（n = 8）为剂量反应，包括添加0%、0.25%、0.5%、1.0%和1.5% MCFA混合物（1：1：1比例C6：0、C8：0和C10：0），以及单独添加0.5% C6：0、C8：0或C10：0的处理。在第0天和第14天从饲喂对照和1.5%MCFA混合饲料的猪收集粪便样品，并使用16 S rDNA测序进行分析。在饲料制造之后，在实验室用PEDV接种之前，将饲料在谷仓温度和湿度下储存在袋中40天。用PEDV接种保留饲料的子样品，以达到10（4）TCID 50/g的滴度，并在接种后0天和3天（dpi）分析单独的样品瓶。总体而言，ADG和ADFI增加（线性，P
An experiment was conducted to evaluate the effect of dietary medium-chain fatty acid (MCFA) addition on nursery pig growth performance, fecal microbial composition, and mitigation of porcine epidemic diarrhea virus (PEDV) following storage. A total of 360 pigs (DNA 400 x 200, Columbus, NE; initially 6.7 +/- 0.07 kg) were randomized to pens (5 pigs per pen) on the day of weaning (approximately 20 d of age), allowed a 6-d acclimation, blocked by BW, and randomized to dietary treatment (9 pens per treatment). All MCFA (Sigma-Aldrich, St. Louis, MO) were guaranteed >= 98% purity, including hexanoic (C6:0), octanoic (C8:0), and decanoic (C10:0) acids. Treatment diets were formulated in 2 phases (7 to 11 and 11 to 23 kg BW) and formulated to meet or exceed NRC requirement estimates. Treatments (n = 8) were a dose response including 0%, 0.25%, 0.5%, 1.0%, and 1.5% added MCFA blend (1:1:1 ratio C6:0, C8:0, and C10:0), as well as treatments with individual additions of 0.5% C6:0, C8:0, or C10:0. Fecal samples were collected from pigs fed control and 1.5% MCFA blend diets on days 0 and 14 and analyzed using 16s rDNA sequencing. Following feed manufacture, feed was stored in bags at barn temperature and humidity for 40 d before laboratory inoculation with PEDV. Subsamples of retained feed were inoculated with PEDV to achieve a titer of 10(4) TCID50/g and separate sample bottles were analyzed on 0 and 3 d post-inoculation (dpi). Overall, ADG and ADFI were increased (linear, P