Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants
Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants
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DOI：
10.1006/viro.1998.9315
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发表时间：
1998-09-30
期刊：
影响因子：
3.7
通讯作者：
Escribano, JM
中科院分区：
文献类型：
--
作者：
Gómez, N;Carrillo, C;Escribano, JM
最近描述了转基因植物作为疫苗生产系统的使用。本文报道了猪传染性胃肠炎冠状病毒糖蛋白S的两种重组形式在转基因植物中表达后所引起的免疫应答。在花椰菜花叶病毒35S(CaMV35S)启动子的控制下，将编码TGEVN-末端结构域(氨基酸残基1-750)或全长糖蛋白S(负责诱导中和抗体)的cDNA转化拟南芥植株。转化植株叶片提取液的基因组DNA和mRNA分析表明，外源基因已整合到拟南芥基因组中，并进行了转录。用特异性抗体进行酶联免疫吸附试验，在大多数转基因植物中观察到重组多肽的表达。用转基因植物叶提取物免疫的小鼠产生了与TGEV特异反应的抗体，免疫沉淀病毒诱导的蛋白，并中和了病毒的感染性。这些结果表明，表达S糖蛋白多肽的转基因植物有可能作为疫苗生产的重组抗原来源。(C)1998年学术出版社。
The use of transgenic plants as vaccine production systems was described recently. We report on the immunological response elicited by two recombinant versions of the glycoprotein S from the swine-transmissible gastroenteritis coronavirus (TGEV) expressed in transgenic plants. Arabidoposis plants were genetically transformed with cDNAs constructs encoding either the N-terminal domain (amino acid residues 1-750) or the full-length glycoprotein S of TGEV, responsible for the neutralizing antibody induction against the virus, under the control of the cauliflower mosaic virus 35S (CaMV 35S) promoter. Genomic DNA and mRNA analyses of leaf extracts from transformed plants demonstrated the incorporation of the foreign cDNA into the arabidopsis genome, as well as their transcription. Expression of recombinant polypeptides were observed in most transgenic plants by ELISA using specific antibodies. Mice immunized with leaf extracts from transgenic plants developed antibodies that reacted specifically with TGEV in ELISA, immunoprecipitated the virus-induced protein, and neutralized the virus infectivity. From these results, we conclude that transgenic plants expressing glycoprotein S polypeptides may possibly be used as a source of recombinant antigen for vaccine production. (C) 1998 Academic Press.