31P MRSI and 1H MRS at 7T: initial results in human breast cancer
31P MRSI and 1H MRS at 7T: initial results in human breast cancer
复制标题
DOI：
10.1002/nbm.1696
复制
发表时间：
2011-12-01
影响因子：
2.9
通讯作者：
Luijten, Peter R.
中科院分区：
文献类型：
--
作者：
Klomp, Dennis W. J.;van de Bank, Bart L.;Luijten, Peter R.
本研究证明了使用高场（7-T）MRI和MRS非侵入性测定人类（乳腺）肿瘤代谢的重要生物标志物的可行性。在此场强下的31 P MRSI用于提供内源性生物标志物的体内检测和定量的直接方法。这些包括磷脂代谢，磷酸盐能量代谢和细胞内pH值。双调谐，双元件收发器的设计与集中的射频场单侧乳腺成像和光谱调谐优化灵敏度在7 T。应用T1加权三维MRI和1H MRS对总胆碱化合物进行定位和定量。每个受试者在20分钟内获得31 P MRSI，并以10 mL的像素体积在乳房上进行三维映射。在新辅助化疗期间，在两名乳腺癌患者中证明了监测体内代谢的可行性，通过离体高分辨率魔角旋转NMR进行验证，并与年龄匹配的健康志愿者的数据进行比较。总胆碱浓度下降到0.4？在体内人乳腺中可检测到mM。腺苷和其他核苷三磷酸，无机磷酸，磷酸胆碱，磷酸乙醇胺和他们的甘油二酯检测腺组织中，以及在肿瘤中的水平，映射到整个乳房。与年龄匹配的健康志愿者相比，在患者中观察到这些化合物的水平改变;在新辅助化疗期间，这些水平发生在乳腺肿瘤中。据我们所知，这是在乳腺癌患者中进行的第一项全面的MRI和MRS研究，该研究揭示了小至10 mL体积的形态学和磷脂代谢的详细信息。这种内源性代谢信息可能为乳腺癌治疗中预后和预测生物标志物的非侵入性评估提供新的方法。版权所有（C）2011约翰威利父子有限公司
This study demonstrates the feasibility of the noninvasive determination of important biomarkers of human (breast) tumor metabolism using high-field (7-T) MRI and MRS. 31P MRSI at this field strength was used to provide a direct method for the in vivo detection and quantification of endogenous biomarkers. These encompass phospholipid metabolism, phosphate energy metabolism and intracellular pH. A double-tuned, dual-element transceiver was designed with focused radiofrequency fields for unilateral breast imaging and spectroscopy tuned for optimized sensitivity at 7 T. T1-weighted three-dimensional MRI and 1H MRS were applied for the localization and quantification of total choline compounds. 31P MRSI was obtained within 20 min per subject and mapped in three dimensions over the breast with pixel volumes of 10 mL. The feasibility of monitoring in vivo metabolism was demonstrated in two patients with breast cancer during neoadjuvant chemotherapy, validated by ex vivo high-resolution magic angle spinning NMR and compared with data from an age-matched healthy volunteer. Concentrations of total choline down to 0.4?mM could be detected in the human breast in vivo. Levels of adenosine and other nucleoside triphosphates, inorganic phosphate, phosphocholine, phosphoethanolamine and their glycerol diesters detected in glandular tissue, as well as in tumor, were mapped over the entire breast. Altered levels of these compounds were observed in patients compared with an age-matched healthy volunteer; modulation of these levels occurred in breast tumors during neoadjuvant chemotherapy. To our knowledge, this is the first comprehensive MRI and MRS study in patients with breast cancer, which reveals detailed information on the morphology and phospholipid metabolism from volumes as small as 10 mL. This endogenous metabolic information may provide a new method for the noninvasive assessment of prognostic and predictive biomarkers in breast cancer treatment. Copyright (C) 2011 John Wiley & Sons, Ltd.