CONCANAMYCIN-A BLOCKS INFLUENZA-VIRUS ENTRY INTO CELLS UNDER ACIDIC CONDITIONS
CONCANAMYCIN-A BLOCKS INFLUENZA-VIRUS ENTRY INTO CELLS UNDER ACIDIC CONDITIONS
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DOI：
10.1016/0014-5793(94)00695-4
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发表时间：
1994-08-08
期刊：
影响因子：
3.5
通讯作者：
CARRASCO, L
中科院分区：
文献类型：
--
作者：
GUINEA, R;CARRASCO, L
液泡质子 ATP 酶的选择性抑制剂刀那霉素 A（如果在病毒感染初期存在的话）可强力阻止流感病毒进入细胞。刀那霉素 A 不会阻止病毒颗粒与细胞的附着，相反，这种大环内酯类抗生素会阻止流感病毒从内体中排出。刀那霉素 A 或尼日利亚霉素在酸性条件下抑制流感病毒进入细胞。此外，如果通过在酸性 pH 值下预孵育细胞来消除 pH 梯度，即使没有任何抑制剂，流感病毒也不会进入。这些结果表明酸性条件本身不足以促进病毒进入细胞；相反，病毒感染的这一步需要pH梯度。
The selective inhibitor of the vacuolar proton-ATPase, concanamycin A, powerfully blocks influenza virus entry into cells, if present during the initial times of virus infection. Attachment of virus particles to cells is not prevented by concanamycin A, rather the exit of influenza virus from endosomes is the step blocked by this macrolide antibiotic. Inhibition of influenza virus entry into cells by concanamycin A or by nigericin takes place under acidic conditions. Moreover, if the pH gradient is abolished by pre-incubation of cells in acidic pH, influenza virus entry does not occur even in the absence of any inhibitors. These results indicate that acidic conditions per se are not sufficient to promote virus entry into cells; rather this step of virus infection requires a pH gradient.