Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes.
Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes.
复制标题
葡萄种子原蛋白素可以改善FB（1）诱导的猪卵母细胞中的减数分裂缺陷。
DOI：
10.3390/toxins13120841
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发表时间：
2021-11-25
期刊：
影响因子：
4.2
通讯作者：
Ju S
中科院分区：
文献类型：
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作者：
Li W;He Y;Zhao H;Peng L;Li J;Rui R;Ju S
伏马菌素B1（FB 1）是伏马菌素中最常见、毒性最大的一种，对人畜健康构成严重威胁。FB 1的细胞毒性与氧化应激和细胞凋亡密切相关。本研究旨在探讨天然抗氧化剂葡萄籽原花青素（GSP）是否能减轻FB 1暴露引起的卵母细胞减数分裂成熟缺陷。在猪卵丘卵母细胞复合体（COCs）体外成熟过程中，分别用30 μM FB 1单独或与100、200和300 μM GSP共处理44 h。结果表明，200 μM GSP可显著改善FB 1的毒性效应，促进第一极体排出，提高随后的卵裂率和囊胚发育率。此外，200 μM GSP共处理恢复了FB 1暴露的卵母细胞的细胞周期进程，减少了异常纺锤体的比例，改善了肌动蛋白分布并保护了线粒体功能。200 μM GSP处理组活性氧（ROS）生成明显减少，CAT、SOD 2和GSH-PX mRNA水平明显升高。GSP处理后，卵母细胞早期凋亡率和自噬水平显著降低，BAX、CASPASE 3、LC 3和ATG 5的mRNA表达水平显著降低，BCL 2和mTOR的mRNA表达水平显著升高。总之，这些结果表明，GSP可以发挥显着的预防作用FB 1诱导的卵母细胞缺陷通过改善氧化应激通过修复线粒体功能障碍。
Fumonisin B1 (FB1), as the most prevalent and toxic fumonisin, poses a health threat to humans and animals. The cytotoxicity of FB1 is closely related to oxidative stress and apoptosis. The purpose of this study is to explore whether Grape seed proanthocyanidin (GSP), a natural antioxidant, could alleviate the meiotic maturation defects of oocytes caused by FB1 exposure. Porcine cumulus oocyte complexes (COCs) were treated with 30 μM FB1 alone or cotreated with 100, 200 and 300 μM GSP during in vitro maturation for 44 h. The results show that 200 μM GSP cotreatment observably ameliorated the toxic effects of FB1 exposure, showing to be promoting first polar body extrusion and improving the subsequent cleavage rate and blastocyst development rate. Moreover, 200 μM GSP cotreatment restored cell cycle progression, reduced the proportion of aberrant spindles, improved actin distribution and protected mitochondrial function in FB1-exposed oocytes. Furthermore, reactive oxygen species (ROS) generation was significantly decreased and the mRNA levels of CAT, SOD2 and GSH-PX were obviously increased in the 200 μM GSP cotreatment group. Notably, the incidence of early apoptosis and autophagy level were also significantly decreased after GSP cotreatment and the mRNA expression levels of BAX, CASPASE3, LC3 and ATG5 were markedly decreased, whereas BCL2 and mTOR were observably increased in the oocytes after GSP cotreatment. Together, these results indicate that GSP could exert significant preventive effects on FB1-induced oocyte defects by ameliorating oxidative stress through repairing mitochondrial dysfunction.