BmBR-C Z4 is an upstream regulatory factor of BmPOUM2 controlling the pupal specific expression of BmWCP4 in the silkworm, Bombyx mori
BmBR-C Z4 is an upstream regulatory factor of BmPOUM2 controlling the pupal specific expression of BmWCP4 in the silkworm, Bombyx mori
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BmBR-C Z4 是 BmPOUM2 的上游调控因子，控制家蚕 Bombyx mori 中 BmWCP4 的蛹特异性表达。
DOI：
10.1016/j.ibmb.2015.09.006
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发表时间：
2015-11-01
影响因子：
3.8
通讯作者：
Feng, Qili
中科院分区：
文献类型：
--
作者：
Deng, Huimin;Niu, Kangkang;Feng, Qili
20-在家蚕中，羟基蜕皮激素（20 E）诱导翅盘角质层蛋白基因BmWCP 4的表达是由转录因子BmPOUM 2介导的，该转录因子与BmWCP 4基因的顺式反应元件（克雷斯）结合。在这项研究中，我们报告了BmPOUM 2的调节。RT-PCR分析表明，BmPOUM 2在蛹前期和蛹中期的表达量最高，BmBR-C Z1、BmBR-C Z2和BmBR-C Z4的表达模式与BmPOUM 2的表达模式一致。BmBR-C Z_4在翅盘中被20 E诱导，而BmBR-C Z_1和BmBR-C Z_2则没有。在BmPOUM 2的启动子区域中鉴定了三个潜在的BR-C Z4顺式反应元件（克雷斯）。BmBR-C Z4在BmN细胞中的过表达可促进BmPOUM 2 mRNA和蛋白的表达，其作用于BmPOUM 2的启动子。电泳迁移率变动试验（EMSA）和BR-C Z4克雷斯野生型和突变体控制下的荧光素酶活性分析表明，BmBR-C Z4蛋白与预测的BRC-Z4 CRE C结合（-684类似于-660）。综上所述，数据表明BmBR-C Z4是BmPOUM 2的直接上游调节因子，并通过BmPOUM 2调节BmWCP 4的蛹特异性表达。(C)2015爱思唯尔有限公司版权所有。
20-hydroxyecdysone (20E)-induced expression of the wing disc cuticle protein gene BmWCP4 was mediated by the transcription factor BmPOUM2, which binds to the cis-response elements (CREs) of BmWCP4 gene in Bombyx mori. In this study we report the regulation of BmPOUM2. RT-PCR analysis indicated that in response to 20E, BmPOUM2 was expressed at higher levels in the wing discs during the pre-pupal and mid-pupal stages than other stages and the expression pattern of BmBR-C Z1, BmBR-C Z2 and BmBR-C Z4 was in tandem with the expression of BmPOUM2. BmBR-C Z4 was induced by 20E in the wing discs, whereas BmBR-C Z1 and BmBR-C Z2 were not. Three potential BR-C Z4 cis-response elements (CREs) were identified in the promoter region of BmPOUM2. The expression of BmPOUM2 mRNA and protein was increased by the over-expression of BmBR-C Z4 in BmN cells, which acted at the promoter of BmPOUM2. Electrophoretic mobility shift assay (EMSA) and the luciferase activity analysis under the control of wild-type and mutants of the BR-C Z4 CREs suggested that BmBR-C Z4 protein bound to the predicted BRC-Z4 CRE C (-684 similar to -660). Taken together the data suggest that BmBR-C Z4 is a direct upstream regulator of BmPOUM2 and regulates the pupal-specific expression of BmWCP4 through BmPOUM2. (C) 2015 Elsevier Ltd. All rights reserved.