Comparison of an rRNA-based and DNA-based nucleic acid amplification test for the detection of Chlamydia trachomatis in trachoma.
Comparison of an rRNA-based and DNA-based nucleic acid amplification test for the detection of Chlamydia trachomatis in trachoma.
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用于检测沙眼中沙眼衣原体的基于 rRNA 和基于 DNA 的核酸扩增测试的比较。
DOI：
10.1136/bjo.2006.099150
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Lietman,ThomasM
中科院分区：
文献类型：
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作者：
Yang,JonL;Schachter,Julius;Moncada,Jeanne;Habte,Dereje;Zerihun,Mulat;House,JenafirI;Zhou,Zhaoxia;Hong,KevinC;Maxey,Kathryn;Gaynor,BruceD;Lietman,ThomasM
背景/目的：世界卫生组织（WHO）希望通过大规模抗生素治疗，在全球范围内消除沙眼，沙眼仍然是全球可预防失明的主要原因。基于DNA的核酸扩增试验（NAAT）目前用于通过测量沙眼衣原体感染的患病率来评估治疗方案的成功。有些人认为，新的核糖体RNA（rRNA）为基础的测试可能更敏感，因为细菌rRNA的存在量高达基因组DNA的10000倍。另一些人认为，基于rRNA的测试灵敏度较低，但更特异，因为存在测试可能无法检测到的死亡或亚活生物体。本研究比较了基于rRNA的测试，以DNA为基础的测试，检测ocularC沙眼感染的儿童生活在沙眼流行villages.Methods：基于rRNA的扩增试验和DNA为基础的聚合酶链反应（PCR）进行拭子标本取自右上跗结膜的56名儿童年龄在0-10岁，生活在两个村庄在阿姆哈拉，Ethiopia.Results：基于rRNA的测试检测ocularC沙眼感染35（63%）的主题相比，22（39%）通过PCR检测（McNemar的测试，p = 0.0002）。 以rRNA为基础的测试得到了阳性结果为所有的主题是阳性的PCR，也检测到感染13（23%）额外的subjects.Conclusion：以rRNA为基础的测试似乎有显着更大的敏感性比PCR检测沙眼流行的村庄儿童眼部衣原体感染。使用基于rRNA的测试，我们可能能够检测到以前PCR遗漏的感染。过去的研究使用基于DNA的检测来评估抗生素治疗后感染性沙眼的患病率，可能低估了感染的真实患病率。
Background/Aim:The World Health Organisation (WHO) hopes to achieve global elimination of trachoma, still the leading cause of preventable blindness worldwide, in part through mass antibiotic treatment. DNA-based nucleic acid amplification tests (NAATs) are currently used to evaluate the success of treatment programmes by measuring the prevalence ofC trachomatisinfection. Some believe that newer ribosomal RNA (rRNA)-based tests may be much more sensitive since bacterial rRNA is present in amounts up to 10 000 times that of genomic DNA. Others believe that rRNA-based tests are instead less sensitive but more specific, due to the presence of dead or subviable organisms that the test may not detect. This study compares an rRNA-based test to a DNA-based test for the detection of ocularC trachomatisinfection in children living in trachoma-endemic villages.Methods:An rRNA-based amplification test and DNA-based polymerase chain reaction (PCR) were performed on swab specimens taken from the right upper tarsal conjunctiva of 56 children aged 0–10 years living in two villages in Amhara, Ethiopia.Results:The rRNA-based test detected ocularC trachomatisinfection in 35 (63%) subjects compared with 22 (39%) detected by PCR (McNemar’s test, p = 0.0002). The rRNA-based test gave positive results for all subjects that were positive by PCR, and also detected infection in 13 (23%) additional subjects.Conclusion:The rRNA-based test appears to have significantly greater sensitivity than PCR for the detection of ocular chlamydial infection in children in trachoma-endemic villages. Using the rRNA-based test, we may be able to detect infection that was previously missed with PCR. Past studies using DNA-based tests to assess prevalence of infectious trachoma following antibiotic treatment may have underestimated the true prevalence of infection.