Revealing the impact of poly(ethylene oxide) blocks on enzyme activable coatings from peptide–polymer conjugates
Revealing the impact of poly(ethylene oxide) blocks on enzyme activable coatings from peptide–polymer conjugates
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DOI：
10.1016/j.eurpolymj.2014.08.014
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发表时间：
2015
影响因子：
6
通讯作者：
Patrick Wilke;H. Börner
中科院分区：
文献类型：
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作者：
Patrick Wilke;H. Börner
酪氨酸酶催化氧化由聚环氧乙烷(PEO)嵌段和贻贝足部蛋白-1(mefp-1)前体片段组成的多肽-聚合物偶联物。在氧化铝表面上观察到从弱/可逆粘结剂到强/不可逆吸附的功能转变。合成了一组PEO-嵌段长度分别为850、3200和5200微克/摩尔的mefp-1-嵌段-PEO生物偶联物，研究了PEO-嵌段长度对氧化铝表面酶活性防污涂层形成的影响。PEO嵌段长度的变化系统地影响酪氨酸酶激活生物偶联物的动力学、活化的生物偶联物的附着行为、所形成的氧化铝涂层的稳定性以及涂层氧化铝表面的防污性能。MEFP-1-BLOCK-PEO3200表现出最好的折衷性能，因为酶的激活和粘接性能表现出良好的性能。在激活状态下，氧化铝表面会形成稳定的涂层，这实际上可以定量地减少白蛋白与蛋白质的相互作用。涂层看起来足够致密和稳定，可以达到与共价“聚乙二醇化”铝衬底类似的防污性能。
Peptide–polymer conjugates composed of a poly(ethylene oxide) (PEO) block and a precursor segment from mussel foot protein-1 (mefp-1) are enzymatically oxidized by tyrosinase. A functional transition from weak/reversible binders to strong/irreversible adsorption onto aluminum oxide surfaces is observed. A set of mefp-1-block-PEO bioconjugates with PEO-block lengths of 850, 3200 and 5200 g/mol is synthesized and investigated to elucidate effects of PEO-block length on the enzyme activable formation of antifouling coatings on aluminum oxide surfaces. The variation of PEO-block length systematically affects the activation kinetics of the bioconjugates by tyrosinase, the adhesion behavior of the activated bioconjugates, the stability of the resulting aluminum oxide coatings and the antifouling properties of coated aluminum oxide surfaces. Mefp-1-block-PEO3200exhibits the best compromise as enzyme activation and adhesive properties showed excellent behavior. Stable coatings on aluminum oxide are formed in the activated state, which reduce albumin protein interactions in a practically quantitative manner. The coating appears to be sufficiently dense and stable to reach similar antifouling properties as covalently “PEGylated” aluminum substrates.