Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells

Plasma elevation of stromal cell-derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells
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DOI：
10.1182/blood.v97.11.3354
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发表时间：
2001-06-01
期刊：
BLOOD
影响因子：
20.3
通讯作者：
Moore, MAS
Moore, MAS
中科院分区：
医学1区
文献类型：
--
作者：
Hattori, K;Heissig, B;Moore, MAS

文献摘要

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趋化因子，基质细胞衍生因子-1（SDF 1），在骨髓中产生，并已被证明通过介导趋化作用和趋化性来调节干细胞归巢至该部位。因此，假设外周循环中SDF 1水平的升高将导致原始造血干细胞和祖细胞的动员。通过将表达SDF 1 α的腺病毒载体（AdSDF 1）静脉内注射到严重联合免疫缺陷小鼠中来增加SDF 1血浆水平。这导致白细胞计数增加10倍，血小板增加3倍，并动员祖细胞，包括集落形成单位-粒细胞-巨噬细胞进入外周循环。此外，AdSDF 1诱导动员具有干细胞潜能的细胞，包括脾中的集落形成单位和长期重建细胞。这些数据表明，SDF 1在外周循环中的过表达会导致具有重建能力的造血细胞、祖细胞和前体细胞的动员，这些研究为在体内研究中使用SDF 1诱导造血干细胞和祖细胞的动员奠定了基础。（血。2001;97：3354-3360）（C）2001由美国血液学学会。
The chemokine, stromal cell-derived factor-1 (SDF1), is produced in the hone marrow and has been shown to modulate the homing of stem cells to this site by mediating chemokinesis and chemotaxis. Therefore, it was hypothesized that elevation of SDF1 level in the peripheral circulation would result in mobilization of primitive hematopoietic stem and progenitor cells. SDF1 plasma level was increased by intravenous injection of an adenoviral vector expressing SDF1 alpha (AdSDF1) into severe combined immunodeficient mice. This resulted in a 10-fold increase in leukocyte count, a 3-fold increase in platelets, and mobilization of progenitors, including colony-forming units-granulocyte-macrophage to the peripheral circulation. In addition, AdSDF1 induced mobilization of cells with stem cell potential, including colony-forming units in spleen and long-term reconstituting cells. These data demonstrate that overexpression of SDF1 in the peripheral circulation results in the mobilization of hematopoietic cells with repopulating capacity, progenitor cells, and precursor cells, These studies lay the foundation for using SDF1 to induce mobilization of hematopoietic stem and progenitor cells in in vivo studies. (Blood. 2001;97:3354-3360) (C) 2001 by The American Society of Hematology.