Expression of early growth response-1 in human gastric cancer and its relationship with tumor cell behaviors and prognosis
Expression of early growth response-1 in human gastric cancer and its relationship with tumor cell behaviors and prognosis
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DOI：
10.1016/j.prp.2013.08.001
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发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Joo, Young-Eun
中科院分区：
文献类型：
--
作者：
Myung, Eun;Park, Young-Lan;Joo, Young-Eun
早期生长反应-1（Egr-1）在与人类癌症进展相关的许多细胞调节过程中至关重要。它的过度表达已在各种人类癌症中得到证实，并可能具有预后意义。本研究的目的是评估Egr-1是否影响人胃癌细胞的侵袭性和致癌性表型，并研究其表达与各种临床病理参数之间的关系，包括人胃癌患者的生存率，我们通过在人胃癌细胞系AGS和TMK 1中使用小干扰RNA研究Egr-1在肿瘤细胞行为中的生物学作用。通过逆转录-聚合酶链反应（RT-PCR）、免疫印迹和免疫组化方法检测Egr-1在人胃癌组织中的表达，发现Egr-1基因的敲低抑制了胃癌细胞AGS和TMK 1的迁移和侵袭。Egr-1在人胃癌和转移淋巴结组织中的表达显著高于正常胃粘膜和非转移淋巴结组织。Egr-1的阳性表达与胃癌的大小、浸润深度、淋巴结转移、分期及生存率有关，提示Egr-1可能通过改变胃癌细胞的迁移、侵袭等行为与胃癌的进展有关。Egr-1的表达可能有助于预测胃癌患者的临床预后。(C)2013年爱思唯尔有限公司。All rights reserved.
The early growth response-1 (Egr-1) is crucial in many cell regulatory processes related to the progression of human cancers. Its overexpression has been demonstrated in variable human cancers and may have prognostic significance. The aims of this current study were to evaluate whether Egr-1 affects invasive and oncogenic phenotypes of human gastric cancer cells, and to examine the relationships between its expression and various clinicopathological parameters, including survival in human gastric cancer patients.We investigated the biologic role of Egr-1 in tumor cell behavior by using a small interfering RNA in human gastric cancer cell lines, AGS and TMK1. The expression of Egr-1 by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry was investigated in human gastric cancer tissues.The knockdown of Egr-1 suppressed tumor cell migration and invasion in AGS and TMK1 cells. Egr-1 expression was significantly increased in human gastric cancer and metastatic lymph node tissues compared to the normal gastric mucosa and non-metastatic lymph node tissues. Positive expression of Egr-1 was significantly associated with tumor size, depth of invasion, lymph node metastasis, tumor stage and poor survival.These results indicate that Egr-1 is associated with human gastric cancer progression through the alteration of tumor cell behavior, such as migration and invasion. Egr-1 expression may help in predicting the clinical outcomes of human gastric cancer patients. (C) 2013 Elsevier GmbH. All rights reserved.