Cajal bodies and the nucleolus are required for a plant virus systemic infection
Cajal bodies and the nucleolus are required for a plant virus systemic infection
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DOI：
10.1038/sj.emboj.7601674
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发表时间：
2007-04-18
期刊：
影响因子：
11.4
通讯作者：
Taliansky, Michael
中科院分区：
文献类型：
--
作者：
Kim, Sang Hyon;Ryabov, Eugene V.;Taliansky, Michael
核仁和Cajal小体(CBS)是重要的相互作用的亚核结构域，参与细胞功能的许多关键方面。某些病毒与这些间隔相互作用，但这种相互作用的功能在很大程度上尚不清楚。在这里，我们证明了花生玫瑰病毒开放阅读框架(ORF)3蛋白通过韧皮部长距离移动病毒RNA的能力严格依赖于它与CBS和核仁的相互作用。ORF3蛋白针对CBS并将其重组为多个CB样结构，然后通过导致这些结构与核仁的融合进入核仁。ORF3蛋白的核仁定位对于随后病毒核糖核蛋白(RNP)颗粒的形成至关重要，RNP颗粒能够使病毒长距离移动和系统感染。我们提供了一个模型，在这个模型中，ORF3蛋白利用涉及CBS的运输途径进入核仁，并与纤维蛋白一起离开细胞核，在细胞质中形成病毒的“有运输能力的”RNP颗粒。
The nucleolus and Cajal bodies (CBs) are prominent interacting subnuclear domains involved in a number of crucial aspects of cell function. Certain viruses interact with these compartments but the functions of such interactions are largely uncharacterized. Here, we show that the ability of the groundnut rosette virus open reading frame (ORF) 3 protein to move viral RNA long distances through the phloem strictly depends on its interaction with CBs and the nucleolus. The ORF3 protein targets and reorganizes CBs into multiple CB-like structures and then enters the nucleolus by causing fusion of these structures with the nucleolus. The nucleolar localization of the ORF3 protein is essential for subsequent formation of viral ribonucleoprotein (RNP) particles capable of virus long-distance movement and systemic infection. We provide a model whereby the ORF3 protein utilizes trafficking pathways involving CBs to enter the nucleolus and, along with fibrillarin, exit the nucleus to form viral 'transport-competent' RNP particles in the cytoplasm.