Linear dendronized polyols as a multifunctional platform for a versatile and efficient fluorophore design
Linear dendronized polyols as a multifunctional platform for a versatile and efficient fluorophore design
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DOI：
10.1039/c8py00193f
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发表时间：
2018-04
影响因子：
4.6
通讯作者：
Ying Li;Katharina Huth;Edzna S Garcia;Benjamin J. Pedretti;Yugang Bai;Gretchen A. Vincil;R. Haag;S. Zimmerman
中科院分区：
文献类型：
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作者：
Ying Li;Katharina Huth;Edzna S Garcia;Benjamin J. Pedretti;Yugang Bai;Gretchen A. Vincil;R. Haag;S. Zimmerman
以开环易位聚合（ROMP）和酸催化脱保护两步反应制备了荧光线性树枝化多元醇（LDPs）。所得的水溶性荧光团尺寸紧凑（<6 nm），并且与先前报道的交联树枝化多元醇（CDP）相比显示出类似的光稳定性，并且与游离荧光团相比显着改善了光稳定性。与CDP的合成相比，LDP的生产需要更少的制备时间、合成工作量和显著更少的Grubbs催化剂。通过引入树枝化单体和荧光团的不同组合，可以进一步微调LDPs的光稳定性和发射波长等物理性质。有趣的是，荧光共振能量转移（FRET）时，观察到两种不同的荧光团被纳入到LDPs。这提供了一种具有大斯托克斯位移的新型荧光团，允许荧光检测同时减少背景重叠。细胞毒性和荧光成像研究证实了这些LDPs的生物相容性，这使它们成为生物应用的潜在候选者。
Fluorescent linear dendronized polyols (LDPs) were prepared in two steps involving a ring-opening metathesis polymerization (ROMP) followed by acid-catalyzed deprotection. The resulting water-soluble fluorophores are compact in size (<6 nm) and show similar photostability compared to previously reported crosslinked dendronized polyols (CDPs) and significantly improved photostability compared to the free fluorophores. In contrast to the synthesis of CDPs, the production of LDPs requires less preparation time, synthetic effort, and significantly less Grubbs catalyst. The photophysical properties, including the photostability and emission wavelength of LDPs, can be further fine-tuned by incorporating different combinations of dendronized monomers and fluorophores. Interestingly, fluorescence resonance energy transfer (FRET) was observed when two different kinds of fluorophores were incorporated into the LDPs. This provides a new type of fluorophore with a large Stokes shift allowing fluorescence detection with reduced background overlap. Cytotoxicity and fluorescence imaging studies confirmed the biocompatibility of these LDPs, which make them potential candidates for biological applications.