Fibronectin matrix composition and organization can regulate cell migration during amphibian development
Fibronectin matrix composition and organization can regulate cell migration during amphibian development
复制标题
DOI：
10.1016/s0925-4773(00)00245-8
复制
发表时间：
2000-04-01
影响因子：
2.6
通讯作者：
Schwarzbauer, JE
中科院分区：
文献类型：
--
作者：
Darribère, T;Schwarzbauer, JE
纤维连接蛋白（FN）是脊椎动物发育所必需的粘附性细胞外基质成分。它在细胞表面形成纤维状基质，控制细胞形态、迁移、增殖和其他重要的细胞过程。为了解决特定功能的FN矩阵结构在早期脊椎动物的发展，我们介绍了正常和突变的重组FN（recFN）到囊胚腔的胚胎的两栖动物Pleurodeles walfl。在这里，我们表明，天然recFN FN FN（A- B-）以及在细胞结合结构域中具有特定突变的recFN，FN（RGD-）和FN（syn-），或在FN结合区域中具有特定突变的recFN，FNL Delta III 1，被组装成纤维状基质。在培养的细胞中形成结构上不同的基质的recFN（FN Delta III 1 -7）在细胞外周组装成聚集体，并且能够以剂量依赖性方式抑制内源性两栖动物FN基质的组装。细胞粘附，扩散和迁移的干扰，在体外和体内的嵌合基质含有FN（RGD-），FN（顺式），或FN三角洲III 1 -7与两栖类FN共组装。在发育上，这种扰动导致中胚层图案化缺陷和原肠胚形成抑制。这些结果表明，FN基质纤维状结构和组成是重要的决定因素的细胞粘附和迁移过程中的发展。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Fibronectin (FN) is an adhesive extracellular matrix component that is essential for vertebrate development. It forms a fibrillar matrix at the cell surface which controls cell morphology, migration, proliferation, and other important cellular processes. To address specific functions of FN matrix structure during early vertebrate development, we introduced normal and mutant recombinant FNs (recFNs) into the blastocoel cavity of embryos of the amphibian Pleurodeles walfl. Here we show that a native recFN FN(A- B-) as well as recFNs with specific mutations in the cell-binding domain, FN(RGD-) and FN(syn-), or in a FN-binding region, FNL Delta III1, are assembled into fibrillar matrix. A recFN (FN Delta III1-7) that forms a structurally distinct matrix in cultured cells was assembled into aggregates at the cell periphery and was able to inhibit assembly of endogenous amphibian FN matrix in a dose-dependent manner. Cell adhesion, spreading, and migration were perturbed in vitro and in vivo on chimeric matrices containing FN(RGD-), FN(syn-), or FN Delta III1-7 co-assembled with amphibian FN. Developmentally, this perturbation resulted in defects in mesoderm patterning and inhibition of gastrulation. These results indicate that FN matrix fibrillar structure and composition are important determinants of cell adhesion and migration during development. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.