RWP-RK domain-containing transcription factors control cell differentiation during female gametophyte development in Arabidopsis.
RWP-RK domain-containing transcription factors control cell differentiation during female gametophyte development in Arabidopsis.
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DOI：
10.1111/nph.14293
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发表时间：
2017-03
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影响因子：
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通讯作者：
F. Tedeschi;Paride Rizzo;T. Rutten;L. Altschmied;H. Bäumlein
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作者：
F. Tedeschi;Paride Rizzo;T. Rutten;L. Altschmied;H. Bäumlein
配子的形成是有性繁殖有机体完成其生命周期的先决条件。在植物中，雌配子是在多细胞组织中形成的，即雌配子体或胚囊。虽然导致雌配子体形成的事件在形态上已经有了特征，但胚囊发育的分子控制仍然难以捉摸。我们使用单突变体和双突变体以及细胞特异性标记系来鉴定拟南芥中一类新的基因调控因子，即RWP-RK结构域(RKD)转录因子。用激光共聚焦扫描和差示干涉对比显微镜进行形态和组织学分析。基因表达和转录组分析分别采用定量逆转录-聚合酶链式反应和RNA测序。我们的结果表明，RKD基因在胚囊发育的不同阶段都有表达。对突变体的形态分析显示，配子体极性和细胞分化严重扭曲。转录组分析表明，几个配子体特异基因家族(RKD2和RKD3)和胚珠发育特异基因(RKD3)的表达发生了变化，并鉴定了对植物激素途径(RKD5)的多效性作用。我们的数据为雌配子体发育的调控提供了新的见解。RKD参与细胞分化的调控，是正常配子体发育所必需的。
The formation of gametes is a prerequisite for any sexually reproducing organism in order to complete its life cycle. In plants, female gametes are formed in a multicellular tissue, the female gametophyte or embryo sac. Although the events leading to the formation of the female gametophyte have been morphologically characterized, the molecular control of embryo sac development remains elusive. We used single and double mutants as well as cell-specific marker lines to characterize a novel class of gene regulators in Arabidopsis thaliana, the RWP-RK domain-containing (RKD) transcription factors. Morphological and histological analyses were conducted using confocal laser scanning and differential interference contrast microscopy. Gene expression and transcriptome analyses were performed using quantitative reverse transcription-PCR and RNA sequencing, respectively. Our results showed that RKD genes are expressed during distinct stages of embryo sac development. Morphological analysis of the mutants revealed severe distortions in gametophyte polarity and cell differentiation. Transcriptome analysis revealed changes in the expression of several gametophyte-specific gene families (RKD2 and RKD3) and ovule development-specific genes (RKD3), and identified pleiotropic effects on phytohormone pathways (RKD5). Our data provide novel insight into the regulatory control of female gametophyte development. RKDs are involved in the control of cell differentiation and are required for normal gametophytic development.