Infective dermatitis has similar immunological features to human T lymphotropic virus-type 1-associated myelopathy/tropical spastic paraparesis.
Infective dermatitis has similar immunological features to human T lymphotropic virus-type 1-associated myelopathy/tropical spastic paraparesis.
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感染性皮炎与人类 T 淋巴细胞病毒 1 型相关脊髓病/热带痉挛性截瘫具有相似的免疫学特征。
DOI：
10.1111/j.1365-2249.2008.03869.x
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发表时间：
2009
影响因子：
4.6
通讯作者：
Carvalho,EM
中科院分区：
文献类型：
--
作者：
Nascimento,MCF;Primo,J;Bittencourt,A;Siqueira,I;deFátimaOliveira,M;Meyer,R;Schriefer,A;Santos,SB;Carvalho,EM
人类嗜T淋巴细胞病毒1型（HTLV-1）是HTLV-1相关脊髓病/热带痉挛性下肢轻瘫（HAM/TSP）、成人T细胞白血病/淋巴瘤和与HTLV-1相关的感染性皮炎（IDH）的病原体。促炎细胞因子的过度产生和HTLV-1前病毒载量的增加是HAM/TSP的特征，但IDH的免疫学基础尚未建立。除了严重的皮肤表现，IDH的重要性依赖于观察到高达30%的IDH儿童在儿童和青少年时期发展HAM/TSP。在这项研究中，我们测定了IDH患者的免疫应答，测量了白细胞介素（IL）-4，IL-5，IL-10，干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α水平以及HTLV-1前病毒载量。此外，向培养物中加入调节性细胞因子和抗细胞因子，以评价这些分子下调TNF-α和IFN-γ合成的能力。HTLV-1携带者和HAM/TSP患者作为对照。IDH组TNF-α和IFN-γ水平高于HTLV-1携带者。IDH组与HAM/TSP组IFN-γ、TNF-α水平无显著性差异。IDH中IL-4 mRNA表达和免疫球蛋白E（IgE）水平有高于HTLV-1携带者的趋势，但差异未达到统计学显著性。IDH患者的HTLV-1前病毒载量显著高于HTLV-1携带者。IDH的特征在于过度的Th 1免疫应答和高HTLV-1前病毒负荷。IDH和HAM/TSP患者的免疫应答之间的相似性以及在IDH中观察到的高前病毒载量支持IDH是HAM/TSP发展的风险因素。
Human T lymphotropic virus-type 1 (HTLV-1) is the causal agent of the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), adult T cell leukaemia/lymphoma and infective dermatitis associated with HTLV-1 (IDH). Over-production of proinflammatory cytokines and an increase in HTLV-1 proviral load are features of HAM/TSP, but the immunological basis of IDH has not been established. In addition to severe cutaneous manifestations, the importance of IDH relies on the observation that up to 30% of children with IDH develop HAM/TSP in childhood and adolescence. In this study we determined the immune response in patients with IDH measuring interleukin (IL)-4, IL-5, IL-10, interferon (IFN)-γ and tumour necrosis factor (TNF)-α levels as well as the HTLV-1 proviral load. Additionally, regulatory cytokines and anti-cytokines were added to cultures to evaluate the ability of these molecules to down-modulate TNF-α and IFN-γ synthesis. HTLV-1 carriers and patients with HAM/TSP served as controls. TNF-α and IFN-γ levels were higher in IDH than in HTLV-1 carriers. There was no difference in IFN-γ and TNF-α concentrations in IDH and HAM/TSP patients. There was a tendency for higher IL-4 mRNA expression and immunoglobulin E (IgE) levels in IDH than in HTLV-1 carriers, but the difference did not reach statistical significance. The HTLV-1 proviral load was significantly higher in IDH patients than in HTLV-1 carriers. IDH is characterized by an exaggerated Th1 immune response and high HTLV-1 proviral load. The similarities between the immunological response in patients with IDH and HAM/TSP and the high proviral load observed in IDH provide support that IDH is a risk factor for development of HAM/TSP.