Hepatocyte growth factor scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells
Hepatocyte growth factor scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells
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DOI：
10.1006/bbrc.1998.8397
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发表时间：
1998-04-07
影响因子：
3.1
通讯作者：
Daikuhara, Y
中科院分区：
文献类型：
--
作者：
Arakaki, N;Kazi, JA;Daikuhara, Y
肝细胞生长因子是目前已知的与散射因子相同的蛋白质，它诱导肉瘤180细胞核DNA的寡核体断裂，并使控制细胞死亡途径的关键成分caspase-3的活性在48小时时增加到对照细胞的2.6倍左右，但不增加caspase-1的活性。与caspase-3抑制剂Ac-DEVD-H共同孵育可完全抑制HGF诱导的DNA断裂和caspase-3活性。相反，HGF不影响凋亡抑制基因Bcl2和Bclx的表达，这些结果表明HGF通过增加Caspase-3的活性而激活了S180细胞的凋亡信号通路。(C)1998年学术出版社。
Hepatocyte growth factor, which is now known to be the same protein as scatter factor, induced oligonucleosomal fragmentation of nuclear DNA of Sarcoma 180 cells and increased the activity of caspase-3, a key component in control of the apoptotic cell death pathway to about 2.6 times that in control cells on 48 hr incubation, but did not increase the activity of caspase-1. Both HGF-induced DNA fragmentation and caspase-3 activity were completely inhibited by co-incubation with an inhibitor of caspase-3, Ac-DEVD-H. In contrast, HGF did not affect the expression of the apoptosis suppressors Bcl-2 and Bcl-x, These results indicate that HGF activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells. (C) 1998 Academic Press.