Early development of the Drosophila brain:: V.: Pattern of postembryonic neuronal lineages expressing DE-cadherin
Early development of the Drosophila brain:: V.: Pattern of postembryonic neuronal lineages expressing DE-cadherin
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DOI：
10.1002/cne.10484
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发表时间：
2003-01-20
影响因子：
2.5
通讯作者：
Hartenstein, V
中科院分区：
文献类型：
--
作者：
Dumstrei, K;Wang, F;Hartenstein, V
果蝇 E-钙粘蛋白同源物 DE-钙粘蛋白由脑神经母细胞及其神经元谱系（“次级谱系”）在胚胎后表达。一旦神经母细胞因体积增大而被识别，DE-钙粘蛋白就会出现在神经母细胞中，然后在整个幼虫生命中不间断地表达。 DE-钙粘蛋白在神经母细胞增殖产生的神经元的细胞体和轴突中保持短暂表达。一般来说，属于一个谱系的神经元的轴突形成紧密的束。这些束的轨迹与它们所属的神经元谱系的位置相关。因此，皮层中相邻的谱系轴突束彼此平行行进，并在相似的位置到达神经桩。因此，可以将连贯的神经母细胞群及其谱系分配给随附文章中介绍的各个神经桩区室和长轴突束（Nassif等人[2003] J Comp Neurol 455:417-434；Younossi-Hartenstein等人[2003] J Comp Neurol 455:435-450)。在这项研究中，我们重建了次级谱系的模式及其与区室和肌成束蛋白 II 阳性长轴突束的关系的投影。根据拓扑结构和轴突轨迹，中枢脑的谱系可细分为 11 个组，可在后续的幼虫阶段中遵循这些组。幼虫谱系图及其轴突投影对于未来果蝇胚胎后神经发生的研究非常重要。它还为研究 DE-钙粘蛋白在幼虫大脑发育中的作用奠定了基础。
The Drosophila E-cadherin homolog, DE-cadherin, is expressed postembryonically by brain neuroblasts and their lineages of neurons ("secondary lineages"). DE-cadherin appears in neuroblasts as soon as they can be identified by their increase in size and then remains expressed uninterruptedly throughout larval life. DE-cadherin remains transiently expressed in the cell bodies and axons of neurons produced by neuroblast proliferation. In general, axons of neurons belonging to one lineage form tight bundles. The trajectories of these bundles are correlated with the location of the neuronal lineages to which they belong. Thus, axon bundles of lineages that are neighbors in the cortex travel parallel to each other and reach the neuropile at similar positions. It is, therefore, possible to assign coherent groups of neuroblasts and their lineages to the individual neuropile compartments and long axon tracts introduced in the accompanying articles (Nassif et al. [2003] J Comp Neurol 455:417-434; Younossi-Hartenstein et al. [2003] J Comp Neurol 455:435-450). In this study, we have reconstructed the pattern of secondary lineages and their projection in relationship to the compartments and Fasciclin II-positive long axon tracts. Based on topology and axonal trajectory, the lineages of the central brain can be subdivided into 11 groups that can be followed throughout successive larval stages. The map of larval lineages and their axonal projection will be important for future studies on postembryonic neurogenesis in Drosophila. It also lays a groundwork for investigating the role of DE-cadherin in larval brain development.