Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress
Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress
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DOI：
10.1104/pp.110.157057
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发表时间：
2010-08-01
期刊：
影响因子：
7.4
通讯作者：
Casati, Paula
中科院分区：
文献类型：
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作者：
Falcone Ferreyra, Maria Lorena;Pezza, Alejandro;Casati, Paula
核糖体蛋白L10（RPL 10）蛋白在植物界中普遍存在。拟南芥（Arabidopsis thaliana）具有编码RPL 10A至RPL 10 C蛋白的三个RPL 10基因，而玉米（Zea mays）基因组中存在两个基因（rpl 10 -1和rpl 10 -2）。玉米和拟南芥RPL 10是组织特异性和发育调控的，在细胞分裂活跃的组织中表现出高水平的表达。免疫共沉淀实验表明，拟南芥中的RPL 10与翻译蛋白相关，表明它是80 S核糖体的一个组成部分。以前，紫外线-B（UV-B）曝光显示，增加了一些玉米核糖体蛋白基因的表达，包括rpl 10。在这项工作中，我们证明，玉米rpl 10基因的诱导UV-B，而拟南芥RPL 10的差异调节这种辐射：RPL 10A是不UV-B调节，RPL 10 B是下调，而RPL 10 C是上调UV-B在所有器官的研究。对拟南芥T-DNA插入突变体的表征表明RPL 10基因在功能上不等同。rpl 10A和rpl 10 B突变体植物表现出不同的表型：敲除rpl 10A突变体是致死的，rpl 10A杂合植物在UV-B条件下翻译缺陷，敲除纯合rpl 10 B突变体显示异常生长。基于这里描述的结果，RPL 10基因不是冗余的，并且参与UV-B胁迫下的发育和翻译。
Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.