Effects of Pulsed Electromagnetic Field Frequencies on the Osteogenic Differentiation of Human Mesenchymal Stem Cells
Effects of Pulsed Electromagnetic Field Frequencies on the Osteogenic Differentiation of Human Mesenchymal Stem Cells
复制标题
脉冲电磁场频率对人间充质干细胞成骨分化的影响
DOI：
10.3928/01477447-20120327-11
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发表时间：
2012-04-01
期刊：
影响因子：
1.1
通讯作者：
Xu, Jianzhong
中科院分区：
文献类型：
--
作者：
Luo, Fei;Hou, Tianyong;Xu, Jianzhong
本研究旨在探讨不同频率脉冲电磁场对人骨髓间充质干细胞成骨分化的影响。用不同频率的脉冲电磁场（包括5、25、50、75、100和150 Hz，场强为1.1 mT）照射第三代人骨髓间充质干细胞，每天30分钟，持续21天。用相差显微镜观察人骨髓间充质干细胞的形态变化。通过检测碱性磷酸酶活性和骨钙素表达来评价人骨髓间充质干细胞的成骨分化，观察不同频率脉冲电磁场对人骨髓间充质干细胞成骨诱导的影响。当脉冲电磁场频率从5 Hz增加到50 Hz时，人间充质干细胞分化水平增加，但当脉冲电磁场频率从50 Hz增加到150 Hz时，效果较弱。在50 Hz时对人骨髓间充质干细胞分化的影响最显著。本研究结果表明脉冲电磁场频率是诱导人骨髓间充质干细胞分化的一个重要因素。此外，50 Hz的脉冲电磁场频率在体外诱导人间充质干细胞成骨细胞分化方面是最有效的。
The purpose of this study was to evaluate the effect of different frequencies of pulsed electromagnetic fields on the osteogenic differentiation of human mesenchymal stem cells. Third-generation human mesenchymal stem cells were irradiated with different frequencies of pulsed electromagnetic fields, including 5, 25, 50, 75, 100, and 150 Hz, with a field intensity of 1.1 mT, for 30 minutes per day for 21 days. Changes in human mesenchymal stem cell morphology were observed using phase contrast microscopy. Alkaline phosphatase activity and osteocalcin expression were also determined to evaluate human mesenchymal stem cell osteogenic differentiation.Different effects were observed on human mesenchymal stem cell osteoblast induction following exposure to different pulsed electromagnetic field frequencies. Levels of human mesenchymal stem cell differentiation increased when the pulsed electromagnetic field frequency was increased from 5 hz to 50 hz, but the effect was weaker when the pulsed electromagnetic field frequency was increased from 50 Hz to 150 hz. The most significant effect on human mesenchymal stem cell differentiation was observed at of 50 hz.The results of the current study show that pulsed electromagnetic field frequency is an important factor with regard to the induction of human mesenchymal stem cell differentiation. Furthermore, a pulsed electromagnetic field frequency of 50 Hz was the most effective at inducing human mesenchymal stem cell osteoblast differentiation in vitro.