OBSERVATIONS ON ESTIMATION OF QUANTITY OF EMPHYSEMA IN LUNGS BY POINT-SAMPLING METHOD
OBSERVATIONS ON ESTIMATION OF QUANTITY OF EMPHYSEMA IN LUNGS BY POINT-SAMPLING METHOD
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DOI：
10.1136/thx.20.5.462
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发表时间：
1965-01-01
期刊：
影响因子：
10
通讯作者：
DUNNILL, MS
中科院分区：
文献类型：
--
作者：
ANDERSON, JA;DUNNILL, MS
在尸检时评估肺气肿的量需要测量存在的异常和正常组织的体积。在先前的论文（Dunnill，1962）中，描述了一种通过简单的点计数技术分别估计肺气肿实质、正常实质以及传导血管和气道（即非实质）体积的方法。该方法需要将肺固定在受控的充气状态下，然后将固定的肺切割成约1 cm。厚片点计数网格，由画在透明塑料材料上的点组成，放置在每个切片上，并记录每个点的位置，即它是否位于正常肺实质、肺气肿实质或非实质上。位于每个成分上的点的总数与每个成分在切片上的面积成比例，而根据Delesse（1847）的定理，切片上的面积又与肺中该成分的体积成比例。在原始文件的点被放置1厘米。这需要为每个肺计算大约2,000到3,000个点。如果要以这种方式定量地评估大量的肺，则需要知道（a）需要计数多少点以达到任何给定的准确度;以及（B）是否需要检查肺的每个切片以获得该肺中肺气肿量的准确测量。例如，如果肺气肿的数量可以通过对肺的一个切片或用Gough和温特沃斯（1949）技术制备的整个肺的一个切片进行点计数来估计，这将是一个明显的优点。这是本文研究的目的。
Assessment of the amount of emphysema in the lungs at necropsy necessitates measurement of the volumes of abnormal and normal tissue present. In a previous paper (Dunnill, 1962) a method was described for estimating separately the volumes of the emphysematous parenchyma, the normal parenchyma, and of the conducting vessels and airways (that is, the non-parenchyma) by a simple point-counting technique. This method entails fixing the lung in a controlled state of inflation and then cutting the fixed lung into approximately 1-cm. thick slices. The point-counting grid, composed of points drawn on a transparent plastic material, is placed over each slice, and the position of each point is noted, ie, whether it liesover normal lung parenchyma, emphysematous parenchyma, or non-parenchyma. The total number of points lying on each component is proportional to the area of each component on the cut slice, which in turn, according to the theorem of Delesse (1847), is proportional to the volume of the component in the lung. In the original paper the points were placed 1 cm. apart, at the angles of equilateral triangles, and this entailed counting some 2,000 to 3,000 points for each lung. If large numbersof lungs are to be assessed quantitatively in this manner, it is desirable to know (a) how many points it is necessary to count to achieve any given degree of accuracy; and (b) whether it is necessary to examine every slice of the lung in order to obtain an accurate measurement of the quantity of emphysema in that lung. It would be an obvious advantage if, for instance, the quantity of emphysema in a lung could be estimated from examination, by point counting, ofa single slice of lung, or of a section of whole lung prepared by the Gough and Wentworth (1949) technique. It is the purpose of this paper to investigate these two questions.