Localization of cyclooxygenase-1 and -2 in adult and fetal human kidney: Implication for renal function
Localization of cyclooxygenase-1 and -2 in adult and fetal human kidney: Implication for renal function
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DOI：
10.1152/ajprenal.1997.272.4.f460
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发表时间：
1997-04-01
影响因子：
4.2
通讯作者：
Nusing, RM
中科院分区：
文献类型：
--
作者：
Komhoff, M;Grone, HJ;Nusing, RM
为了解环氧化酶（考克斯）-1和-2在人肾脏中的作用，我们分析了它们在成人和胎儿正常肾脏中的表达和定位。免疫组织化学显示考克斯-1在集合管细胞、间质细胞、内皮细胞和肾小球前、后血管平滑肌细胞中均有表达。考克斯-2免疫反应阳性蛋白表达于动脉和静脉的内皮细胞和平滑肌细胞以及肾小球内的足细胞。与大鼠相反，在致密斑中未检测到考克斯异构体。这些数据证实了原位mRNA分析使用地高辛标记的核糖核酸探针。考克斯-1在胎肾中主要表达于足细胞和集合管细胞。考克斯-1在两种细胞中的表达水平从被膜下到髓质皮质均显著增加。肾小球考克斯-2染色仅在肾发育末期的足细胞中检测到。总之，考克斯-2的定位表明，这种酶可能主要参与肾灌注和肾小球血流动力学的调节。考克斯-1在胎儿肾脏足细胞中的表达和在成人肾小球中的缺失表明，这种亚型可能参与肾小球的发生。
To gain insight into the roles of cyclooxygenase (COX)-1 and -2 in human kidney, we analyzed their expressions and localization in adult and fetal normal kidney Immunohistology showed expression of COX-1 in collecting duct cells, interstitial cells, endothelial cells, and smooth muscle cells of pre- and post-glomerular vessels. Expression of COX-2 immunoreactive protein could be localized to endothelial and smooth muscle cells of arteries and veins and intraglomerularly in podocytes. In contrast to the rat, COX isoforms were not detected in the macula densa. These data were confirmed by in situ mRNA analysis using digoxigenin-labeled riboprobes. In fetal kidney, COX-1 was primarily expressed in podocytes and collecting duct cells. Expression levels of COX-1 in both cell types increased markedly from subcapsular to juxtamedullary cortex. Glomerular staining of COX-2 was detectable in podocytes only at the endstage of renal development. In summary, the localization of COX-2 suggests that this enzyme may be primarily involved in the regulation of renal perfusion and glomerular hemodynamics. The expression of COX-1 in podocytes of the fetal kidney and its absence in adult glomeruli suggests that this isoform might be involved in glomerulogenesis.