Transcranial electric stimulation entrains cortical neuronal populations in rats.
Transcranial electric stimulation entrains cortical neuronal populations in rats.
复制标题
DOI：
10.1523/jneurosci.5252-09.2010
复制
发表时间：
2010-08-25
期刊：
影响因子：
--
通讯作者：
Buzsáki G
中科院分区：
文献类型：
--
作者：
Ozen S;Sirota A;Belluscio MA;Anastassiou CA;Stark E;Koch C;Buzsáki G
在体外和人体研究中，低强度电场被认为会影响正在进行的神经元活动。然而，弱电场如何影响完整的大脑活动并与之相互作用的生理机制还不是很清楚。我们在体内进行了麻醉大鼠新皮质和海马区的细胞外和细胞内记录，以及行为正常大鼠的细胞外记录。通过放置在颅骨表面或硬脑膜表面的电极，以低频率(0.8、1.25或1.7赫兹)的正弦模式产生电场。经颅电刺激(TES)可靠地将神经元缠绕在广泛的皮质区域，包括海马区。TES锁相神经元的百分比随着刺激强度的增加而增加，并与动物的行为状态有关。在记录部位，TES诱发的电压梯度低至1 mV/mm，足以产生相位偏置的神经元尖峰。细胞内记录显示，刺激和阈值下活动都受到TES强迫场和网络活动的共同影响。我们认为，慢性制剂中的TES可用于脑活动的实验和治疗控制。
Low intensity electric fields have been suggested to affect the ongoing neuronal activity in vitro and in human studies. However, the physiological mechanism of how weak electrical fields affect and interact with intact brain activity is not well understood. We performed in vivo extracellular and intracellular recordings from the neocortex and hippocampus of anaesthetized rats and extracellular recordings in behaving rats. Electric fields were generated by sinusoid patterns at slow frequency (0.8, 1.25 or 1.7 Hz) via electrodes placed on the surface of the skull or the dura. Transcranial electric stimulation (TES) reliably entrained neurons in widespread cortical areas, including the hippocampus. The percentage of TES phase-locked neurons increased with stimulus intensity and depended on the behavioral state of the animal. TES-induced voltage gradient, as low as 1 mV/mm at the recording sites, was sufficient to phase-bias neuronal spiking. Intracellular recordings showed that both spiking and subthreshold activity were under the combined influence of TES forced fields and network activity. We suggest that TES in chronic preparations may be used for experimental and therapeutic control of brain activity.