Structural features of the single-stranded DNA-binding protein MoSub1 from Magnaporthe oryzae
Structural features of the single-stranded DNA-binding protein MoSub1 from Magnaporthe oryzae
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来自 Magnaporthe oryzae 的单链 DNA 结合蛋白 MoSub1 的结构特征。
DOI：
10.1107/s0907444912019932
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发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Peng, Youliang
中科院分区：
文献类型：
--
作者：
Huang, Jinguang;Zhao, Yanxiang;Peng, Youliang
转录辅助因子Sub1/PC 4在转录过程中具有多种功能。它在转录起始中起着积极和消极的作用，并参与延伸和下游转录过程，并作为转录再起始因子。MoSub 1是来自稻瘟病菌的Sub1/PC 4直向同源物，其与单链DNA dT（12）紧密结合，亲和力为186 nM。计算了MoSub_1的晶体结构，分辨率达到1.79 μ m。该蛋白的结构与PC 4的结构高度相似，并且具有与PC 4相似的二聚体界面和DNA结合区域，表明MoSub 1可以使用与Sub 1/PC 4家族的其他蛋白相同的基序结合DNA。MoSub 1结构中有两个新的特征：DNA结合结构域的N端区域和C端延伸。MoSub 1的DNA结合结构域的N-末端区域向后转向DNA结合位点，并且可以直接与DNA或DNA结合位点相互作用。PC 4中不存在的C-末端延伸区可能无法与DNA相互作用，这可能是Sub1和PC 4之间差异的一个原因。
The well studied general transcription cofactor Sub1/PC4 has multiple functions in transcription. It plays both a negative and a positive role in transcription initiation and is involved in elongation and downstream transcription processes and as a transcription reinitiation factor. MoSub1, a Sub1/PC4 orthologue from rice blast fungus, binds the single-stranded DNA dT(12) tightly with an affinity of 186 nM. The crystal structure of MoSub1 has been solved to 1.79 Å resolution. The structure of the protein shows high similiarity to the structure of PC4 and it has a similar dimer interface and DNA-binding region to PC4, indicating that MoSub1 could bind DNA using the same motif as other proteins of the Sub1/PC4 family. There are two novel features in the MoSub1 structure: a region N-terminal to the DNA-binding domain and a C-terminal extension. The region N-terminal to the DNA-binding domain of MoSub1 turns back towards the DNA-binding site and may interact directly with DNA or the DNA-binding site. The C-terminal extension region, which is absent in PC4, may not be capable of interacting with DNA and is one possible reason for the differences between Sub1 and PC4.