Bone marrow-derived CX3CR1 progenitors contribute to neointimal smooth muscle cells via fractalkine CX3CR1 interaction
Bone marrow-derived CX3CR1 progenitors contribute to neointimal smooth muscle cells via fractalkine CX3CR1 interaction
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DOI：
10.1096/fj.09-132225
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发表时间：
2010-01-01
期刊：
影响因子：
4.8
通讯作者：
Caplice, Noel M.
中科院分区：
文献类型：
--
作者：
Kumar, Arun H. S.;Metharom, Pat;Caplice, Noel M.
平滑肌细胞在许多血管疾病中起着重要作用，有助于重塑、损伤后修复和动脉形成，这些细胞的来源被认为位于血管壁和循环血液中。目前，血管外平滑肌祖细胞（SPCs）的确切起源和分化机制尚不清楚。我们在此表明，小鼠骨髓中的CX(3)CR1单核细胞群为血管损伤后新内膜斑块内的平滑肌细胞提供了SPCs的来源。此外，体内CX(3)CR1-fractalkine （FKN）相互作用在血管壁水平上对骨髓源性祖细胞的平滑肌细胞分化至关重要。骨髓来源的CX(3)CR1阳性细胞与FKN相互作用的功能能力在一定程度上也是血管损伤后新内膜形成的关键。最后，在骨髓来源的CX(3)CR1阳性细胞的纯制备中，我们发现在FKN的存在下，体外平滑肌细胞分化明显增加。我们的数据强调了髓系和血管系统之间的一种新的功能关系，并在血管损伤和修复的背景下强调了一种关键的趋化因子受体途径，当平滑肌细胞分化需要时，它可能调节细胞命运。-库马尔，A. H. S, Metharom， P., Schmeckpeper， J., Weiss， S., Martin， K., capplice， N. M.骨髓来源的CX(3)CR1祖细胞通过fractalkine CX(3)CR1相互作用对新内膜平滑肌细胞的影响。中国生物医学工程学报（英文版），2010(1)。www.fasebj.org
Smooth muscle cells play a major role in numerous vascular diseases that contribute to remodeling, repair after injury, and arteriogenesis, and the source of these cells is thought to lie within the vessel wall and the circulating blood. Currently, the precise origin and mechanism of differentiation of extravascular smooth muscle progenitor cells (SPCs) is unclear. We show here that the CX(3)CR1 mononuclear cell population of murine bone marrow provides a source of SPCs that contributes to smooth muscle cells within the neointimal plaque after vascular injury. Moreover, CX(3)CR1-fractalkine (FKN) interaction in vivo is essential for smooth muscle cell differentiation of bone marrow-derived progenitor cells at the vessel wall level. Functional competence of bone marrow-derived CX(3)CR1 positive cells to interact with FKN is also crucial in part for neointima formation following vascular injury. Finally, in a pure preparation of bone marrow-derived CX(3)CR1 positive cells, we show that in vitro smooth muscle cell differentiation increases markedly in the presence of FKN. Our data highlight a novel functional relationship between the myeloid and vascular systems and in the context of vascular injury and repair underscores a key chemokine-receptor pathway that may regulate cell fate when smooth muscle cell differentiation is required.-Kumar, A. H. S., Metharom, P., Schmeckpeper, J., Weiss, S., Martin, K., Caplice, N. M. Bone marrow-derived CX(3)CR1 progenitors contribute to neointimal smooth muscle cells via fractalkine CX(3)CR1 interaction. FASEB J. 24, 81-92 (2010). www.fasebj.org