Influence of Methamphetamine on Genital Herpes Simplex Virus Type 2 Infection in a Mouse Model
Influence of Methamphetamine on Genital Herpes Simplex Virus Type 2 Infection in a Mouse Model
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DOI：
10.1097/olq.0b013e31825af129
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发表时间：
2012-09-01
影响因子：
3.1
通讯作者：
Bourne, Nigel
中科院分区：
文献类型：
--
作者：
Valencia, Frances;Bubar, Marcy J.;Bourne, Nigel
背景：兴奋剂甲基苯丙胺的使用越来越普遍，全世界有3500万吸毒者。兴奋剂的使用与艾滋病毒和其他性传播感染(STI)发病率的增加之间存在已知的联系。冰毒已知具有免疫调节作用。然而，冰毒对正常免疫反应和性传播感染疾病发病机制的影响还没有得到充分的研究。方法：我们使用一个具有良好特征的小鼠模型来研究冰毒对生殖器单纯疱疹病毒2型感染的影响。空斑分析和实时定量聚合酶链式反应检测病毒复制。应用细胞因子微球阵列和酶联免疫吸附试验检测宿主天然免疫应答过程中细胞因子的水平。结果：冰毒治疗改变了患者的行为、临床体征和疾病进展。冰毒处理的小鼠的阴道上皮变薄，感觉神经节中的病毒增加。此外，冰毒引起了局部细胞因子分泌的失调，而对全身细胞因子分泌的影响微乎其微。结论：宿主免疫反应的甲基化改变部分地促进了生殖器疱疹疾病的进展。这些发现将提高对冰毒使用对宿主免疫反应和疾病易感性的理解。
Background: Use of the stimulant methamphetamine (METH) is increasingly common, with >35 million users worldwide. There is a known association between stimulant use and an increased incidence of HIV and other sexually transmitted infections (STIs). METH is known to have immune modulatory properties. However, the impact of METH on normal immune responses and disease pathogenesis with STIs has not been fully examined.Methods: We used a well-characterized murine model to investigate the impact of METH use on genital herpes simplex virus type 2 infection. Plaque assay and quantitative real-time polymerase chain reaction were used to measure viral replication. Cytokine bead array and enzyme-linked immunosorbent assay were used to determine levels of cytokines during host innate immune response.Results: METH treatment altered behavior, onset of clinical signs, and disease progression. METH-treated mice also had a thinned vaginal epithelium and an increase in virus present in the sensory ganglia. In addition, METH produced a local dysregulation of cytokine secretion that contrasts with its minimal impact on systemic cytokine secretion.Conclusions: Results suggest that the METH alterations of the host immune response partially contribute to enhanced genital herpes disease progression. These findings will improve understanding of METH use on host immune responses and susceptibility to disease.