Orthogonal Injection Ion Funnel Interface Providing Enhanced Performance for Selected Reaction Monitoring-Triple Quadrupole Mass Spectrometry.
Orthogonal Injection Ion Funnel Interface Providing Enhanced Performance for Selected Reaction Monitoring-Triple Quadrupole Mass Spectrometry.
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DOI：
10.1021/acs.analchem.5b01482
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发表时间：
2015-07-21
影响因子：
7.4
通讯作者：
Smith RD
中科院分区：
文献类型：
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作者：
Chen TC;Fillmore TL;Prost SA;Moore RJ;Ibrahim YM;Smith RD
电动力离子漏斗有助于在质谱仪的高压区域（例如离子源界面）高效聚焦和转移带电粒子，从而提高灵敏度。一种“离轴”离子漏斗设计已经被开发出来，以减少源污染和干扰，例如ESI液滴残留物和其他低聚焦的中性或带电粒子具有非常高的质量电荷比。本研究建立了由正交高压电动力离子漏斗（HPIF）和离子漏斗阱结合三重四极杆质谱仪组成的双离子漏斗界面，并对其进行了表征。在9 ~ 10 Torr的压力下，采用正交离子注入入口和排斥板电极将离子导入离子漏斗HPIF。影响HPIF性能的关键因素包括射频幅值、直流梯度和操作压力的影响。与三重四极杆标准界面相比，低丰度肽直接定量质谱分析的检出限提高了4倍以上。对加入高度复杂混合物的低丰度肽进行液相色谱选择反应监测（LC-SRM）分析的灵敏度增强也与使用商用s透镜界面和在线双离子漏斗界面获得的灵敏度进行了比较。
The electrodynamic ion funnel facilitates efficient focusing and transfer of charged particles in the higher-pressure regions (e.g., ion source interfaces) of mass spectrometers, thus providing increased sensitivity. An “off-axis” ion funnel design has been developed to reduce the source contamination and interferences from, e.g. ESI droplet residue and other poorly focused neutral or charged particles with very high mass-to-charge ratios. In this study, a dual ion funnel interface consisting of an orthogonal higher pressure electrodynamic ion funnel (HPIF) and an ion funnel trap combined with a triple quadrupole mass spectrometer was developed and characterized. An orthogonal ion injection inlet and a repeller plate electrode was used to direct ions to an ion funnel HPIF at a pressure of 9–10 Torr. Key factors for the HPIF performance characterized included the effects of RF amplitude, the DC gradient, and operating pressure. Compared to the triple quadrupole standard interface more than 4-fold improvement in the limit of detection for the direct quantitative MS analysis of low abundance peptides was observed. The sensitivity enhancement in liquid chromatography selected reaction monitoring (LC-SRM) analyses of low-abundance peptides spiked into a highly complex mixture was also compared with that obtained using both a commercial S-lens interface and an in-line dual-ion funnel interface.