Factors associated with obesity alter matrix remodeling in breast cancer tissues.
Factors associated with obesity alter matrix remodeling in breast cancer tissues.
复制标题
与肥胖相关的因素改变乳腺癌组织中的基质重塑。
DOI：
10.1117/1.jbo.25.1.014513
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发表时间：
2020
影响因子：
3.5
通讯作者：
Georgakoudi,Irene
中科院分区：
文献类型：
--
作者：
Zhang,Yang;Baloglu,FatmaKucuk;Ziemer,LaurenEHillers;Liu,Zhiyi;Lyu,Boyang;Arendt,LisaM;Georgakoudi,Irene
肥胖与患乳腺癌的风险较高以及所有年龄段女性的疾病结局较差有关。乳腺组织基质的组成、密度和组织也已知在疾病的发展和进展中起重要作用。然而，肥胖和间质重塑之间的联系还不清楚。我们试图表征从暴露于正常或高脂肪（肥胖诱导）饮食的小鼠获得的健康和癌性乳腺组织内胶原基质的详细组织特征。我们进行了二次谐波产生和光谱双光子激发荧光成像，我们提取了胶原相关荧光（CAF）的水平沿着胶原含量，三维和二维组织的度量。正常组织和肿瘤组织之间的CAF强度和整体胶原组织存在显著差异;然而，肥胖增强了这些指标的变化，特别是当考虑三维组织指标时。因此，我们的研究表明，肥胖症的影响显着胶原组织和结构和相关的通讯途径可能是重要的未来治疗目标。
Obesity is associated with a higher risk of developing breast cancer and with worse disease outcomes for women of all ages. The composition, density, and organization of the breast tissue stroma are also known to play an important role in the development and progression of the disease. However, the connections between obesity and stromal remodeling are not well understood. We sought to characterize detailed organization features of the collagen matrix within healthy and cancerous breast tissues acquired from mice exposed to either a normal or high fat (obesity inducing) diet. We performed second-harmonic generation and spectral two-photon excited fluorescence imaging, and we extracted the level of collagen-associated fluorescence (CAF) along with metrics of collagen content, three-dimensional, and two-dimensional organization. There were significant differences in the CAF intensity and overall collagen organization between normal and tumor tissues; however, obesity-enhanced changes in these metrics, especially when three-dimensional organization metrics were considered. Thus, our studies indicate that obesity impacts significantly collagen organization and structure and the related pathways of communication may be important future therapeutic targets.