Protective effect of sialyllactose on the intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli challenge
Protective effect of sialyllactose on the intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli challenge
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DOI：
10.1039/d2fo02066a
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发表时间：
2022-10-10
期刊：
影响因子：
6.1
通讯作者：
He, Jun
中科院分区：
文献类型：
--
作者：
Duan, Qiming;Yu, Bing;He, Jun
唾液酸乳糖 (SL) 是猪母乳中最丰富的低聚糖之一，与许多生物功能有关，包括益生元和免疫调节作用。本研究旨在研究猪模型中膳食 SL 补充剂对接触产肠毒素大肠杆菌 (ETEC) 的肠道屏障功能的影响。 32头猪被分配到4个处理组，饲喂基础或含SL（5.0 g kg(-1)）日粮，并口服ETEC或培养基。补充SL显着降低了腹泻发生率和粪便中大肠杆菌的丰度（P < 0.05）。有趣的是，SL 减轻了 ETEC 诱导的肠上皮损伤，如二胺氧化酶 (DAO) 和 d-乳酸血清浓度降低所示，并减少了空肠上皮中凋亡细胞的数量 (P < 0.05)。此外，SL不仅提高了十二指肠和回肠上皮中紧密连接蛋白ZO-1的丰度，而且还提高了ETEC攻击后空肠上皮中的抗氧化能力和SIgA阳性细胞的数量（P < 0.05）。重要的是，在 ETEC 攻击后，SL 降低了十二指肠和回肠中炎症相关基因的表达水平，例如肿瘤坏死因子-α (TNF-α)、白介素-6 (IL-6)、活化 B 细胞核因子 kappa 轻链增强子 (NF-kappa B)、骨髓分化因子 88 (MyD88) (P < 0.05)。 SL 还显着提高空肠中两种关键抗氧化基因的表达水平，例如核因子红细胞 2 相关因子 2 (Nrf-2) 和 kelch 样 ECH 相关蛋白 1 (KEAP-1) (P < 0.05)。这些结果表明，SL可以减轻ETEC引起的肠上皮损伤，这与抑制炎症、提高肠道免疫力、抗氧化能力和改善肠上皮功能有关。
Sialyllactose (SL), one of the most abundant oligosaccharides present in porcine breast milk, has been implicated in many biological functions, including the prebiotic and immune-modulating effects. This study was conducted to investigate the influences of dietary SL supplementation on intestinal barrier functions exposure to enterotoxigenic Escherichia coli (ETEC) in a porcine model. Thirty-two pigs were assigned to four treatments, fed with basal or SL-containing (5.0 g kg(-1)) diet, and orally infused with ETEC or culture medium. SL supplementation significantly reduced the diarrhea incidence and the abundance of E. coli in feces (P < 0.05). Interestingly, SL attenuated ETEC-induced intestinal epithelium injury as indicated by the decreased serum concentrations of diamine oxidase (DAO) and d-lactate and reduced the number of apoptotic cells in the jejunal epithelium (P < 0.05). Moreover, SL not only elevated the abundance of the tight-junction protein ZO-1 in the duodenal and ileal epithelium but also elevated the antioxidant capacity and the number of SIgA positive cells in the jejunal epithelium upon the ETEC challenge (P < 0.05). Importantly, SL decreased the expression levels of inflammation-related genes such as the tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B), myeloid differentiation factor 88 (MyD88) in the duodenum, and ileum upon ETEC challenge (P < 0.05). SL also significantly elevated the expression levels of two critical antioxidant genes such as the nuclear factor erythroid-2 related factor 2 (Nrf-2) and kelch-like ECH-associated protein 1 (KEAP-1) in the jejunum (P < 0.05). These results suggested that SL can alleviate ETEC-induced intestinal epithelium injury, which is associated with suppressed inflammation, improved intestinal immunity, antioxidant capacity, and improved intestinal epithelial functions.