Identification of dinucleoside polyphosphates by matrix-assisted laser desorption/ionisation post-source decay mass spectrometry
Identification of dinucleoside polyphosphates by matrix-assisted laser desorption/ionisation post-source decay mass spectrometry
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DOI：
10.1002/rcm.1035
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Schlüter, H
中科院分区：
文献类型：
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作者：
Jankowski, J;Grosse-Hüttmann, P;Schlüter, H
二核苷多磷酸是一组细胞内和细胞外介质，控制许多生理功能。在这项研究中，二核苷多磷酸正离子基质辅助激光解吸/电离飞行时间质谱（MADLI-TOFMS）检查。3-羟基吡啶甲酸被用作紫外吸收基质。对于单个二核苷多磷酸Ap（n）A（n = 2-7）、Ap（n）G（n = 2-6）和Gp（n）G（n = 2-6），测量MALDI源后衰变（PSD）质谱。MALDI-PSD质谱中的每个质量峰可以归属于二核苷多磷酸的单个片段。本文所示的二核苷多磷酸的裂解模式的比较表明，二核苷多磷酸优选裂解成由相应的单核苷多磷酸以及相应的核苷和碱基组成的碎片离子，其在MALDI质谱仪的无场漂移路径中飞行期间。因此，本文所述的MALDI-PSD方法适用于鉴定其他二核苷多磷酸。本MALDI-PSD质谱可用作MALDI-PSD质量参考谱，用于将来鉴定二核苷多磷酸和其他核苷酸。版权所有（C）2003约翰威利父子有限公司。
Dinucleoside polyphosphates are a group of intra- and extracellular mediators controlling numerous physiological functions. In this study dinucleoside polyphosphates were examined by positive ion matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MADLI-TOFMS). 3-Hydroxypicolinic acid was used as UV-absorbing matrix. For the individual dinucleoside polyphosphates Ap(n)A (n = 2-7), Ap(n)G (n = 2-6) and Gp(n)G (n = 2-6), MALDI post-source decay (PSD) mass spectra were measured. Each mass peak in the MALDI-PSD mass spectra could be assigned to individual fragments of dinucleoside polyphosphates. The comparison of the fragmentation patterns of the dinucleoside polyphosphates presented here demonstrates that dinucleoside polyphosphates preferably cleave to fragment ions consisting of the corresponding monortucleoside polyphosphates as well as the corresponding nucleosides and bases during flight in the field-free drift path of the MALDI mass spectrometer. Therefore, the MALDI-PSD approach described here is suitable for identification of other dinucleoside polyphosphates. The present MALDI-PSD mass spectra may be used as MALDI-PSD mass reference spectra for future identification of dinucleoside polyphosphates and other nucleotides. Copyright (C) 2003 John Wiley Sons, Ltd.