Gallic acid, a major component of Toona sinensis leaf extracts, contains a ROS-mediated anti-cancer activity in human prostate cancer cells
Gallic acid, a major component of Toona sinensis leaf extracts, contains a ROS-mediated anti-cancer activity in human prostate cancer cells
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DOI：
10.1016/j.canlet.2009.05.040
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发表时间：
2009-12-28
期刊：
影响因子：
9.7
通讯作者：
Yuan, Shyng-Shiou
中科院分区：
文献类型：
--
作者：
Chen, Huei-Mei;Wu, Yang-Chang;Yuan, Shyng-Shiou
前列腺癌是美国老年男性中最常见的恶性肿瘤，已成为亚洲的一个主要健康问题。以往的研究已证明香椿叶提取物具有较高的药用价值。对包括前列腺癌在内的多种癌细胞具有细胞毒活性。在本研究中，没食子酸是冬虫夏草叶提取物中的主要抗癌成分。它通过产生活性氧(ROS)和线粒体介导的细胞凋亡而对DU145前列腺癌细胞产生细胞毒作用，而这两种作用可被抗氧化剂过氧化氢酶和N-乙酰半胱氨酸逆转。此外，没食子酸通过激活Chk1和Chk2，抑制CDc25C和CDc2的活性，将DU145细胞的生长阻断在G2/M期。此外，没食子酸与阿霉素有协同抑制DU145细胞生长的作用。综上所述，我们的结果表明没食子酸有可能被开发成一种抗前列腺癌的药物，值得进一步研究。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Prostate cancer, the most frequently diagnosed malignancy in elderly males of the United States, has become a major health issue in Asia. Previous studies have demonstrated that leaf extracts of Toona sinensis Roem. contain cytotoxic activity on several cancer cells including prostate cancer cells. In this study, gallic acid is identified as the major anti-cancer compound in T. sinensis leaf extracts. It is cytotoxic to DU145 prostate cancer cells, through generation of reactive oxygen species (ROS) and mitochondria-mediated apoptosis, which were reversed by antioxidants catalase and N-acetylcysteine. Furthermore, gallic acid is shown to block the growth of DU145 cells at G2/M phases by activating Chk1 and Chk2 and inhibiting Cdc25C and Cdc2 activities. In addition, gallic acid has a synergistic effect with doxorubicin in suppressing the growth of DU145 cells. Taken together, our results suggest that gallic acid has the potential to be developed into an anti-prostate cancer drug and is worthy of further studies. (C) 2009 Elsevier Ireland Ltd. All rights reserved.