Expression of interleukin-18 in human atherosclerotic plaques and relation to plaque instability
Expression of interleukin-18 in human atherosclerotic plaques and relation to plaque instability
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DOI：
10.1161/hc3901.096721
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发表时间：
2001-10-02
期刊：
影响因子：
37.8
通讯作者：
Tedgui, A
中科院分区：
文献类型：
--
作者：
Mallat, Z;Corbaz, A;Tedgui, A
背景-白细胞介素（IL）-18是一种具有潜在致动脉粥样硬化特性的强效促炎细胞因子。它的表达和动脉粥样硬化中的作用，然而，是unknow.Methods和Results-In本研究中，我们研究了稳定和不稳定的人颈动脉粥样硬化斑块检索动脉内膜切除术的IL-18的存在下，使用逆转录-聚合酶链反应（PCR），蛋白质印迹和免疫组化技术。与对照正常动脉相比，IL-18在动脉粥样硬化斑块中高度表达，并且主要定位于斑块巨噬细胞中。IL-18受体在斑块巨噬细胞和内皮细胞中也上调，提示潜在的生物学效应。为了研究IL-18在动脉粥样硬化中的作用，我们使用实时定量PCR确定了IL-18 mRNA表达与斑块不稳定迹象之间的关系。有趣的是，在有症状（不稳定）的斑块中IL-18 mRNA水平显著高于无症状（稳定）的斑块（P
Background-Interleukin (IL)-18 is a potent proinflammatory cytokine with potential atherogenic properties. Its expression and role in atherosclerosis, however, are unknown.Methods and Results-In the present study, we examined stable and unstable human carotid atherosclerotic plaques retrieved by endarterectomy for the presence of IL-18 using reverse transcription-polymerase chain reaction (PCR), Western blot, and immunohistochemical techniques. IL-18 was highly expressed in the atherosclerotic plaques compared with control normal arteries and was localized mainly in plaque macrophages. IL-18 receptor was also upregulated in plaque macrophages and endothelial cells, suggesting potential biological effects. To examine the role of IL-18 in atherosclerosis, we determined the relation between IL-18 mRNA expression and signs of plaque instability using real-time quantitative PCR. Interestingly, significantly higher levels of IL-18 mRNA were found in symptomatic (unstable) plaques than asymptomatic (stable) plaques (P