Intravenous grafts recapitulate the neurorestoration afforded by intracerebrally delivered multipotent adult progenitor cells in neonatal hypoxic-ischemic rats.
Intravenous grafts recapitulate the neurorestoration afforded by intracerebrally delivered multipotent adult progenitor cells in neonatal hypoxic-ischemic rats.
复制标题
静脉移植物概括了新生儿低氧 - 缺血性大鼠中脑部递送的多能祖细胞提供的神经修养。
DOI：
10.1038/jcbfm.2008.68
复制
发表时间：
2008-11
影响因子：
6.3
通讯作者：
Borlongan, Cesar V.
中科院分区：
文献类型：
--
作者：
Yasuhara, Takao;Hara, Koichi;Maki, Mina;Mays, Robert W.;Deans, Robert J.;Hess, David C.;Carroll, James E.;Borlongan, Cesar V.
一旦缺氧缺血性（HI）损伤在人类新生儿出生时发生，所产生的脑损伤持续整个个体的一生，因为目前没有改善的治疗方法可用。我们最近发现，脑内移植多能成体祖细胞（MAPCs）的结果在新生大鼠HI损伤模型的行为改善和减少缺血性细胞丢失。为了将这种细胞疗法推向临床，我们探索了MAPCs更实用和侵入性更小的静脉内给药。7日龄的Sprague-Dawley大鼠最初受到单侧HI损伤，然后7天后接受同种异体大鼠MAPCs的脑内或静脉内注射。在移植后第7天和第14天，与仅接受溶媒输注的动物相比，通过脑内或静脉途径接受MAPC的动物表现出运动和神经评分改善。移植后第14天的免疫组化评价显示，脑内和静脉内移植的MAPCs在缺血海马区检测到。海马细胞的保存程度在两个处理组中几乎相同，并且大于溶剂组。这些结果表明MAPCs的静脉内递送是具有临床应用潜力的可行且有效的细胞疗法。
Once hypoxic–ischemic (HI) injury ensues in the human neonate at birth, the resulting brain damage lasts throughout the individual’s lifetime, as no ameliorative treatments are currently available. We have recently shown that intracerebral transplantation of multipotent adult progenitor cells (MAPCs) results in behavioral improvement and reduction in ischemic cell loss in neonatal rat HI-injury model. In an attempt to advance this cellular therapy to the clinic, we explored the more practical and less invasive intravenous administration of MAPCs. Seven-day-old Sprague–Dawley rats were initially subjected to unilateral HI injury, then 7 days later received intracerebral or intravenous injections of allogeneic rat MAPCs. On post-transplantation days 7 and 14, the animals that received MAPCs via the intracerebral or intravenous route exhibited improved motor and neurologic scores compared with those that received vehicle infusion alone. Immunohistochemical evaluations at day 14 after transplantation revealed that both intracerebrally and intravenously transplanted MAPCs were detected in the ischemic hippocampal area. The degree of hippocampal cell preservation was almost the same in the two treatment groups and greater than that in the vehicle group. These results show that intravenous delivery of MAPCs is a feasible and efficacious cell therapy with potential for clinical use.
登录
查看更多内容
影响因子：
8.3
作者：
Borlongan, CV;Hadman, M;Sanberg, PR
通讯作者：
Sanberg, PR
新生儿缺氧 - 缺血性损伤后，大脑中的反应性星形胶质细胞简要调节神经细胞和血管细胞趋化因子SDF-1（CXCL12）表达。
影响因子：
2.4
作者：
Miller, JT;Bartley, JH;Wimborne, HJC;Walker, AL;Hess, DC;Hill, WD;Carroll, JE
通讯作者：
Carroll, JE
影响因子：
3.3
作者：
Yasuhara, Takao;Matsukawa, Noriyuki;Borlongan, Cesar V.
通讯作者：
Borlongan, Cesar V.
影响因子：
1.7
作者：
Borlongan, CV;Tajima, Y;Sanberg, PR
通讯作者：
Sanberg, PR
影响因子：
5.3
作者：
Jansen, EM;Solberg, L;Low, WC
通讯作者：
Low, WC