Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis)
Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis)
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南方鲶 (Silurus meridionalis) 性别连锁 DNA 标记的筛选和表征以及标记辅助选择
DOI：
10.1016/j.aquaculture.2019.734783
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发表时间：
2020-02-25
期刊：
影响因子：
4.5
通讯作者：
Wang, Deshou
中科院分区：
文献类型：
--
作者：
Zheng, Shuqing;Wang, Xiaoshuang;Wang, Deshou
南方鲶（Silurus meridionalis）具有两性二态性，雌性生长速度快于雄性，是中国重要的经济鱼类。然而，缺乏快速、准确的性别特异性标记阻碍了性别控制在育种过程中的应用以及该物种性别决定的研究。本研究通过对雄性和雌性样本的下一代测序（NGS）和重测序数据进行比较分析，筛选出38条候选Y染色体特异性序列，并通过PCR扩增在南方鲶中验证其性别特异性。最终获得8个性别连锁DNA标记，用于区分南方鲶鱼的遗传性别。重要的是，其中一个标记 (Marker-8) 可以作为高度特异性和稳健的共显性标记，用于 XX、XY 和 YY 个体的遗传性别鉴定。随后，开发了标记辅助选择（MAS）技术，用于大规模生产用于水产养殖的XX全雌性和用于基础研究（特别是性别决定）的YY超级雄性。总之，我们目前的数据表明南方鲶鱼存在 XY 性别决定系统。本研究获得的性连锁DNA标记对于南方鲶性别决定基因的分离、性别决定机制的阐明以及加速南方鲶性别控制育种的发展具有潜在的应用价值。
The Southern catfish (Silurus meridionalis), which exhibits a sexual dimorphism with female growth faster than male, is an economically important fish in China. However, the lack of a rapid and accurate sex specific marker has hampered the application of sex control in the breeding process, as well as the investigations of sex-determination in this species. In this study, by comparative analysis of next generation sequencing (NGS) and resequencing data from male and female samples, 38 candidate Y chromosome-specific sequences were screened out, their sex-specificity were validated in Southern catfish through PCR amplification. Finally, 8 sex-linked DNA markers were obtained to distinguish the genetic sex of Southern catfish. Importantly, one of those markers (Marker-8) could serve as a highly specific and robust co-dominant marker for genetic sex identification of XX, XY and YY individuals. Subsequently, a marker-assisted selection (MAS) technique was developed for the mass production of XX all-females for aquaculture and YY supermales for basic research especially in sex determination. In conclusion, our current data suggest an XY sex determination system in the Southern catfish. Sex-linked DNA markers obtained in this study will have potential applications in the isolation of sex determine gene, elucidation of the sex determination mechanism, and accelerate the development of sex control breeding in Southern catfish.